Effects of Vitamin C on Oxidative Stress Due to Anesthesia and Surgical Trauma in Dogs

다국어 초록 (Multilingual Abstract)

The study evaluated the antioxidant effects of ascorbic acid (AA) against oxidative stress during laparotomy in dogs under general anesthesia using isoflurane. Twelve dogs were randomly assigned to either the ascorbic acid group (AAG) or the sham group (SG). AAG received intravenous dosage of 100 mg/animal of standard AA 10 minutes before anesthesia. Plasma levels of cortisol, glucose, total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI) were measured. Cortisol level increased significantly with time in both groups (p < 0.05). The change of glucose levels was not significantly different between both groups. TOS and OSI increased significantly with time in the SG (p < 0.05), whereas it did not significantly change with time in the AAG. The TOS and OSI of the AAG were significantly lower than in the SG (p < 0.05). However, TAS did not significantly change between both groups. The results suggest that the decrease of TOS in the AAG might be related to the conversion of antioxidants to oxidants. The decrease of OSI indicates that the decrease of reactive oxygen species (i.e., oxidative stress) produced at the site of injury is able to decrease surgical side effects of abnormal blood circulation, organ failure, and inflammation. Therefore, AA can be used to protect a surgical patient from oxidative stress in canine laparotomy.

참고문헌 (Reference)

1 Fukushima R, "Vitamin C requirement in surgical patients" 13 : 669-676, 2010

2 Berger MM, "Update on clinical micronutrient supplementation studies in the critically ill" 9 : 711-716, 2006

3 Günel E, "Treatment of intestinal reperfusion injury using antioxidative agents" 33 : 1536-1539, 1998

4 Koksal H, "Total oxidant status, total antioxidant status, and paraoxonase and arylesterase activities during laparoscopic cholecystectomy" 65 : 285-290, 2010

5 Egwu GO, "The effect of vitamin C at varying times on physiological parameters in rabbits after xylazine anaesthesia" 47 : 97-104, 2011

6 De Vries AP, "Supplementation with anti-oxidants Vitamin C and E decreases cyclosporine A trough-levels in renal transplant recipients" 21 : 231-232, 2006

7 Trachootham D, "Redox regulation of cell survival" 10 : 1343-1374, 2008

8 Gutteridge J, "Redox imbalance in the critically ill" 55 : 49-75, 1999

9 Nathens AB, "Randomized, prospective trial of antioxidant supplementation in critically ill surgical patients" 236 : 814-822, 2002

10 Bartels M, "Protective effect of antioxidative vitamins against lipid peroxidation in liver ischemia and reperfusion - an animal experimental study" 6 : 152-166, 2007

11 Sasaki M, "Oxidative stress and ischemia-reperfusion injury in gastrointestinal tract and antioxidant, protective agents" 40 : 1-12, 2007

12 Caryl Goodyear-Bruch, "Oxidative Stress in Critically Ill Patients" 11 : 543-551, 2002

13 Kohen R, "Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification" 30 : 620-650, 2002

14 Tsuchiya M, "Open abdominal surgery increases intraoperative oxidative stress: can it be prevented" 107 : 1946-1952, 2008

15 Marana E, "Neuroendocrine stress response in patients undergoing benign ovarian cyst surgery by laparoscopy, minilaparotomy, and laparotomy" 10 : 159-165, 2003

16 Buyukhatipoglu H, "N-acetylcysteine fails to prevent renal dysfunction and oxidative stress after noniodine contrast media administration during percutaneous coronary interventions" 120 : 383-389, 2010

17 Abdullahi Elsa, "Ketamine anaesthesia following premedication of rabbits with vitamin C" 대한수의학회 6 (6): 239-241, 2005

18 Sa söz N, "Ischaemia-reperfusion injury of rat ovary and the effects of vitamin C, mannitol and verapamil" 17 : 2972-2976, 2002

19 Lagowska-Lenard M, "Influence of vitamin C on markers of oxidative stress in the earliest period of ischemic stroke" 62 : 751-756, 2010

20 Behrendt D, "Impact of coronary endothelial function on the progression of cardiac transplant-associated arteriosclerosis: effect of anti-oxidant vitamins C and E" 25 : 426-433, 2006

21 Papich MG, "Handbook of veterinary drugs. 1st ed" Saunders 31-, 2002

22 Baysal Z, "Evaluation of total oxidative and antioxidative status in pediatric patients undergoing laparoscopic surgery" 44 : 1367-1370, 2009

23 Ryu HY, "Effects of vitamin C as antioxidant on recovery of left ventricular function after ischemia and reperfusion in isolated rat heart" 593-598, 1996

24 Pirbudak L, "Effect of ascorbic acid on surgical stress response in gynecologic surgery" 58 : 928-931, 2004

25 Dodam JR, "Duration of etomidate-induced adrenocortical suppression during surgery in dogs" 51 : 786-788, 1990

26 Jae-il Lee, "Attenuation of ischemia-reperfusion injury by ascorbic acid in the canine renal transplantation" 대한수의학회 7 (7): 375-379, 2006

27 Bulger EM, "Antioxidants in critical illness" 136 : 1201-1207, 2001

28 Cuzzocrea S, "Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia / reperfusion injury" 53 : 135-159, 2001

29 Ullegaddi R, "Antioxidant supplementation enhances antioxidant capacity and mitigates oxidative damage following acute ischaemic stroke" 59 : 1367-1373, 2005

30 Van Stijn MF, "Antioxidant enriched enteral nutrition and oxidative stress after major gastrointestinal tract surgery" 14 : 6960-6969, 2008

31 Erel O, "A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation" 37 : 277-285, 2004