Original Articles : Selection of a l-Lysine-Overproducing Strain of the Red Seaweed Porphyra suborbiculata (Rhodophyta) through Mutation and Analog Enrichment = Original Articles : Selection of a l-Lysine-Overproducing Strain of the Red Seaweed Porphyra suborbiculata (Rhodophyta) through Mutation and Analog Enrichment

다국어 초록 (Multilingual Abstract)

An improved strain of the red seaweed Porphyra suborbiculata containing an increased amount of the essential amino acid llysine was obtained through mutation and analog enrichment. Mutagenesis using a 10% lethal dose of ultraviolet irradiation and an enrichment culture with the l-lysine analog aminoethyl-l-cysteine (AEC) was repeated to select the most productive strain using monospores of P. suborbiculata. The concentrations of AEC required to produce 50 and 100% inhibition of survival were 60 and 115mM in the parent strain, and 72 and 135mM in the selected AEC-resistant strain, respectively. The AEC-resistant strain, L130, produced 1.74-fold more lysine compared to its parent strain. Thus, mutagenesis with analog enrichment shows promise for selecting seaweed strains that can overproduce this essential amino acid.

참고문헌 (Reference)

1 Kronick MN, "The use of phycobiliproteins as fluorescent labels in immunoassay" 92 : 1-13, 1986

2 Moustafa RAK, "The effect of gamma radiation and N-ethyl-N-nitrosourea on cultured maize callus growth and plant regeneration" 17 : 121-132, 1989

3 Tseng CK, "Studies on the alternation of the nuclear phases of chromosome numbers in the life history of some species of Porphyra from China" 32 : 1-8, 1989

4 Elvevoll EO, "Seafood diets: hypolipidemic and antiatherogenic effects of taurine and n-3 fatty acids" 200 : 396-402, 2008

5 Queener SW, "Screening and selection for strain improvement. In: Manual of Industrial Microbiology and Biotechnology" American Society for Microbiology 155-169, 1986

6 Hawkes MW, "Reproductive strategies. In: Biology of the Red Algae" Cambridge University Press 455-476, 1990

7 Fujita Y, "Protoplast isolation and fusion in Porphyra (Bangiales, Rhodophyta)" 204 : 161-166, 1990

8 Lowry OH, "Protein measurement with the Folin phenol reagent" 193 : 265-275, 1951

9 Sahoo D, "Porphyra: the economic seaweed as a new experimental system" 83 : 1313-1316, 2002

10 Saga N, "Porphyra: a model plant in marine sciences" 68 : 1075-1078, 2002

11 Young VR, "Plant proteins in relation to human protein and amino acid nutrition" 59 : 1203-1212, 1994

12 Association of Official Analytical Chemists, "Official Methods of Analysis" Association of Official Analytical Chemists 2005

13 Jin LG, "Morphology and sequence analysis of nuclear 18S rDNA from the summer strain of Porphyra suborbiculata (Rhodophyta) in Korea" 33 : 489-495, 2000

14 Provasoli L, "Media and prospects for the cultivation of marine algae. In: Cultures and Collections of Algae" Japanese Society of Plant Physiologists 63-75, 1968

15 Bhattacharjee JK, "Lysine biosynthesis in eukaryotes. In: Amino Acids: Biosynthesis and Genetic Regulation" Addition-Wesley Publishing Co. 1983

16 Tosaka O, "Lysine In: Biotechnology of Amino Acid Production" Kodansha Ltd 152-172, 1986

17 Tosaka O, "L-lysine production by S-(2- aminoethyl) L-cysteine and α-amino-β-hydroxyvaleric acid resistant mutants of Brevibacterium lactofermentum" 42 : 745-752, 1978

18 Korea Fisheries Association, "Korean Fisheries Yearbook" Samshin Publishing Co 2010

19 Schrumpf B, "Isolation and prominent characteristics of an L-lysine hyperproducing strain of Corynebacterium glutamicum" 37 : 566-571, 1992

20 Rowlands RT, "Industrial strain improvement: mutagenesis and random screening procedures" 6 : 3-10, 1984

21 Noda H, "Health benefits and nutritional properties of nori" 5 : 255-258, 1993

22 Japanese Society of Fisheries, "Genetics and Breeding of Aquatic Organism" Kouseisha Kouseikaku 1979

23 Carlton BC, "Gene mutation. In: Manual of Methods for General Bacteriology" American Society for Microbiology 222-242, 1981

24 Radin NS, "Extraction of tissue lipids with a solvent of low toxicity" 72 : 5-7, 1981

25 Zhu CJ, "Determination of biomass dry weight of marine microalgae" 9 : 189-194, 1997

26 Cahyanto MN, "Construction of Lactobacillus plantarum strain with enhanced Llysine yield" 102 : 674-679, 2007

27 Cole KM, "Chromosomes. In: Biology of the Red Algae" Cambridge University Press 73-101, 1990

28 Kochert G, "Carbohydrate determination by the phenol sulfuric acid method. In: Handbook of Phycological Methods. Vol. II. Physiological and Biochemical Methods" Cambridge University Press 95-97, 1978

29 Garrett RH, "Biochemistry. 3rd ed" Thomson Brooks/Cole, Inc. 2005

30 Brown LM, "Applications of genetics to microalgae production" 31 : 281-274, 1990

31 Kisumi M, "Application of intracellular genetic recombination to strain construction. In: Biotechnology of Amino Acid Production" Kodansha Ltd. 14-23, 1986

32 Kagawa A, "Amino acid composition in Japanese foods. In: Standard Tables of Food Composition in Japan" Resource Council of STA 253-259, 1983