Callus induction and browning suppression in tree peony Paeonia ostii ‘Fengdan’

다국어 초록 (Multilingual Abstract)

Callus induction is an important stage in micropropagation. In this study, embryos, cotyledons, and hypocotyls of treepeony ( Paeonia ostii ‘Fengdan’) were used as explants to induce callus formation. Callus induction was largely infl uencedby the medium, plant growth regulator, and explant. All combinations of the medium and plant growth regulator wereconducive to callus induction from zygotic embryos, where the ratio of explants with callus induction was very high in allof the combinations. The greatest ratio of explants with callus induction from the cotyledon explants was found on WoodyPlant Medium (WPM) or Murashige and Skoog (MS) medium supplemented with both 0.5 mg L −1 thidiazuron (TDZ)and either 0.5 mg L −1 2,4-dichlorophenoxyacetic acid (2,4-D) or 0.5 mg L −1 1-naphthaleneacetic acid (NAA), and on theWPM containing 0.5 mg L −1 NAA and 0.5 mg L −1 6-benzylaminopurine (BA). The ratio of explants with callus inducedfrom the hypocotyl explants was the greatest on either MS medium or WPM supplemented with both 0.5 mg L −1 2,4-D and0.5 mg L −1 TDZ. In contrast, a combination of 0.5 mg L −1 2,4-D and 0.5 mg L −1 TDZ, or 0.5 mg L −1 NAA and 0.5 mg L −1TDZ was highly likely to cause a browning problem during culture. For browning suppression, calcium chloride (CaCl 2 ),polyvinyl pyrrolidone (PVP), gallic acid, and caff eic acid were much more eff ective than other reagents. The effi cacy onbrowning suppression was concentration-dependent, and the best results were obtained at a concentration of 4.0 mg L −1CaCl 2 , 1.0–2.0 g L −1 PVP, 0.5–1.0 mg L −1 gallic acid, and 0.5–1.0 mg L −1 caff eic acid, respectively. In summary, callusformation was successfully induced from the zygotic embryo, cotyledon, and hypocotyl explants, and callus browning waseff ectively suppressed by caff eic acid (0.5–1.0 mg L −1 ), gallic acid (1.0 mg L −1 ), CaCl 2 (4.0 mg L −1 ), and PVP (1.0 g L −1 ).

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