Corynebacterium sp. K-199가 생산하는 단백질성 생물용집제 = A Proteinous Bioflocculant Produced by Corynebacterium sp. K-199

국문 초록 (Abstract)

미생물응집제를 개발하기 위하여 자연계로부터 방선균 600여 균주와 보관균주를 대상으로 응집활성이 우수한 균주를 선별하고 동정한 결과 corynebacteium 속으로 동정되었다. 응집제 생산을 위한 최적조건은 2% glycerol, 0.4% peptone, 0.1% yeast extract, 0.3% K_2HPO_4, 0.1% CaCO_3, 0.05% NaCl, 0.1% MgSO_4·7H_2O, pH 7.5의 배지 100 ml을 500ml baffle flask에 넣고 96시간 회전진탕배양할 때였으며 상기의 조건으로 jar fermentor 배양시 96시간에서 780 units/ml의 응집활성을 나타내었다. 응집물질은 pronase 처리와 periodate 산화에 의해 각각 무처리구와 13%와 106%의 활성을 나타냄으로써 응집활성은 주로 단백질에 기이함을 알 수 있다.

다국어 초록 (Multilingual Abstract)

About 600 microorganisms isolated form soil, marsh, compost, etc. were examined for their flocculating ability in the kaolin suspension and swine wastewater. Among them, K-199 was the best producer of flocculant and was identified to be a species belong to the genus corynebacteium. The maximum production of the flocculant from Corynebacterium sp. K-199 was observed in culture medium containing 2% glycerol, 0.4% peptone, 0.3% K_2HPO_4, 0.1% CaCO_3, 0.05% NaCl, 0.1% yeast extract, 0.1% MgSO_4·7H_2O and initial pH 7.5 when cultured with rotary shaker controlled at 25℃ and 150 rpm. Under the optimal culture condition with jar fermentor, the maximum production was reached to flocculating activity of 780 units/ml after 4 days. From the results of the activity be fully maintained by periodate oxidization, it suggests that the activity is due to the protein.