어병세균 Edwardsiella tarda에 대한 한약재 추출물의 항균활성 = Antibacterial Activity of Herbal Medicine Extracts against Edwardsiella tarda

다국어 초록 (Multilingual Abstract)

The methanol extracts of 19 commercial herb medicines was analyzed to antibacterial activities against Edwardsiella tarda, causing several fish diseases. Rhus javanica showed most strong antibacterial activity against E. tarda and Escherichia coli. Methanol extract of R. javanica was further extracted using several organic solvents having different polarity. Extract from ethyl acetate fraction showed strong activity against E. tarda as well as E. coli. Minimal inhibitory concentration, MIC of R. javanica extract was measured and resulted showing 64 ㎍/㎖ for E. tarda and 256 ㎍/㎖ for E. coli. It is needed that, from these results, further purification and isolation of reposible compound of these activities and further study on the synergy effect using combination with antibiotics against pathogenic bacteria.

참고문헌 (Reference)

1 최혜승, "오배자 추출물의 어병 세균에 대한 항균 효능" 한국어병학회 18 (18): 239-245, 2005

2 허정호, "경남 남부지역 양식어류 질병에서 항생제 감수성에 관한 연구" 한국임상수의학회 19 (19): 19-22, 2002

3 이성규, "가축질병 균주에 대한 소목의 항균활성" 한국미생물·생명공학회 31 (31): 242-249, 2003

4 최일, "Staphylococcus aureus와 Salmonella gallinarum에 대한 한약재의 항균활성" 한국미생물·생명공학회 30 (30): 177-183, 2002

5 최일, "Staphylococcus aureus 에 대한 한약재의 항균활성" 한국자원식물학회 19 (19): 491-6, 2006

6 Kim JS, "Spatial and temporal occurrence of Edwardsiella tarda at flounder farms in Jeju" 19 : 173-181, 2001

7 Lee BW, "Screening of natural antimicrobial plant extract on food spoilage microorganism" 23 : 200-204, 1991

8 Han JS, "Isolation and antimicrobial action of growth inhibitory substance on food-borne microorganisms from Dryopteris crassirhizoma Nakai" 33 : 611-618, 2001

9 Lee EW, "Functional cloning and expression of emeA, and characterization of EmeA, a multidrug efflux pump from Enterococcus faecalis" 26 : 266-270, 2003

10 Edward JN, "Fish disease Diagnosis and treatment" Iowa state university press 367-, 2000

11 황의경, "Escherichia coli에 대한 한약재추출물의 항균활성" 대한수의학회 43 (43): 625-631, 2003

12 Jung SC, "Current report on the antibiotic using in animals and the antibiotic resistant bacteria" 40 : 144-149, 2008

13 Treves-Brown KM, "Applied fish pharmacology" Kluwer Academic publishers 309-, 2000

14 Beuchat IR, "Antimicrobials occuring naturally in foods" 43 : 134-139, 1989

15 Mitscher LA, "Antimicrobial agents from higher plants, antimicrobial isoflavonoids and related substances from Glycyrrhiza glabra L. var Typica" 43 : 259-269, 1980

16 Lee MC, "Antimicrobial activity of extract from gall-nut and red-grape husk" 10 : 174-179, 1997