Characterization and phylogenetic analysis of chitin synthase genes from the genera Sporobolomyces and Bensingtonia subrorea

다국어 초록 (Multilingual Abstract)

We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification fromgenomic DNAs of four strains of the genus Sporobolomycesand of Bensingtonia subroseausingdegenerated primers based on conserved regions of the CHS genes. Though amino acid sequencesof these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences weredifferent in size, which was due to various introns present in seven fragments. Alignment andphylogenetic analysis of their deduced amino acid sequences together with the reported CHSgenes of basidiomycetes separated the sequences into classes I, II and III. This analysis alsopermitted the classification of isolated CHSs; SgCHS1belongs to class I, bAelInd IcelIndIrelIvd INrelIn , and SsCHS1belong to class II, and BsCHS2belongs to class III. The deducedamino acid sequences involving in class II that were discovered from five strains were alsocompared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysisand phylogenetic tree by neighborjoining method revealed the taxonomic and evolutionaryposition for four strains of the genus Sporobolomycesand for Bensingtonia subroseawhich agreedwith the previous classification. The results clearly showed that CHS fragments could be used as avaluable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.

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