P-41 Comparison of eicosanoid metabolite profiles in plasma between controlled and refractory asthmatics = P-41 Comparison of eicosanoid metabolite profiles in plasma between controlled and refractory asthmatics

다국어 초록 (Multilingual Abstract)

Background: Leukotrienes and prostaglandins are known to be involved in development and progression of asthma. However, other metabolites in eicosanoids pathways have not been revealed in terms of asthma severity. In this study, we tried to identify eicosanoid metabolite associated with refractory asthma. Methods: 110 Plasma of 57 controlled and 45 refractory asthmatics and 6 normal subjects were used in this study, which were obtained from a biobank of Soonchunhyang University Bucheon Hospital. Forty three eicosanoid metabolites in the samples were analyzed using LC-MS/MS. Results: In plasma samples, 29 of 43 eicosanoids were detected, which were higher in asthmatics than in normal controls. Among them, the levels of PGJ2 (metabolized by COS), 5-oxo-ETE, 12-oxo-ETE, LXA4, 5-HETE, LTB4 (metabolized by LOX) and 8(9)-DHET (metabolized by CYP-sEH) were significantly higher in refractory asthma than those in controlled asthma. Conclusion: Inflammatory mediators such as 5-HETE and 5-oxo-ETE were elevated in refractory asthma, which suggest that the metabolites play key roles in pathophysiology. However, anti-inflammatory metabolites such as LXA4 also increase in refractory asthma, which may be protection against the development of the disease. Although further replication and systemic functional study should be necessary, we discovered refractory asthma-related eicosanoids, which may be useful to understand underlying mechanisms of refractory asthma. [funded by 2015-ER7402-00)].