Genetic Introduction of Foreign Genes to Pleurotus eryngii by Restriction Enzyme-Mediated Integration

다국어 초록 (Multilingual Abstract)

Pleurotus eryngii was transformed via restriction enzyme-mediated integration. In order to construct the transformation plasmid, the enhanced cyan fluorescent protein (ECFP) gene was ligated next to the gpd promoter of the plasmid pAN7-1. Transformation was facilitated via the heat treatment of a transformation mixture containing 1 μg of the HindIII-digested plasmid DNA and 106 mushroom protoplasts in 40%polyethyleneglycol solution, resulting in 10-40 hygromycin-resistant transformants. Successful transformation was evidenced by PCR, Southern blot, and confocal fluorescence microscopic analyses on the selected transformants. To date, this is the first report on the transformation of P. eryngii by REMI technique.

참고문헌 (Reference)

1 Kim, K., "Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration" 209 : 273-276, 2002

2 Punt, P.J., "Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli" 56 : 117-124, 1987

3 Joong-HoJoh, "The Efficient Transformation of Pleurotus ostreatus using REMI Method" 한국균학회 31 (31): 32-35, 2003

4 Bölker, M., "Tagging pathogenicity genes in Ustilago maydis by restriction enzyme-mediated integration (REMI)" 248 : 547-552, 1995

5 Kuspa, A., "Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA" 89 : 8803-8807, 1992

6 Ruiz-Diez, B., "Strategies for the transformation of filamentous fungi" 92 : 189-195, 2002

7 Irie, T., "Stable transformation of Pleurotus ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals" 56 : 707-709, 2001

8 Michielse, C.B., "Role of bacterial virulence proteins in Agrobacterium-mediated transformation of Aspergillus awamori" 41 : 571-578, 2004

9 van Peer, A.F., "Phleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune" 75 : 1243-1247, 2009

10 Gómez-Toribio, V., "Induction of extracellular hydroxyl radical production by white-rot fungi through quinone redox cycling" 75 : 3944-3953, 2009

11 Wang, B., "Improved phytoremediation of oilseed rape (Brassica napus) by Trichoderma mutant constructed by restriction enzyme-mediated integration (REMI) in cadmium polluted soil" 74 : 1400-1403, 2009

12 Kim, S., "Genetic transformation and mutant isolation in Ganoderma lucidum by restriction enzymemediated integration" 233 : 201-204, 2004

13 SHIN, EUN-HYE, "Biodegradation of Endocrine-disrupting Bisphenol A by White Rot FungusIrpex lacteus" 한국미생물·생명공학회 17 (17): 1147-1151, 2007

14 de Groot, M.J., "Agrobacterium tumefaciens-mediated transformation of filamentous fungi" 16 : 839-842, 1998

15 Fan, L., "Advances in mushroom research in the last decade" 44 : 303-311, 2006

16 Zorn, H., "A peroxidase from Lepista irina cleaves beta,beta-carotene to flavor compounds" 384 : 1049-1056, 2003

17 Chen, X., "A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus" 66 : 4510-4513, 2000

18 Rosa Elena Cardoza, "A Comparison of the Phenotypic and Genetic Stability of Recombinant Trichoderma spp. Generated by Protoplast- and Agrobacterium-Mediated Transformation" 한국미생물학회 44 (44): 383-395, 2006