Solanum commersonii에서 도입된 감자의 풋마름병 저항성 표지인자의 추적과 야생종 중에서 역병저항성 개체의 선발에 관한 연구 = Studies on the bacteria wilt resistant marker from S. commersonii and selection of late blight resistant clones among potato related solanum species

다국어 초록 (Multilingual Abstract)

The purpose of this study was to test Late-blight resistancy for the wild-solanum species, and selected cell fusion hybrids between the bacterial wilt resistant clone of S.commersonii and a haploid of potato cultivar superior and the sexual cross progenies of the fusion hybrids. In vitro plants were used for resistancy test. Inoculation of the P. infestans on the leaves were 5㎕ per leaf with a concentration 2.5∼3.0×10³zoospore/ ml.
The fusion parents had high sensitivity to two race of P.infestans tested. High level of restance to P.infestans appeared in some of the F₁ progenies, somatic hybrid. Therefore, it seemed not to be related with resistant genetic factor. S. bulbocastanum PI 243510-1, PI 243510-3, S. pinnatisectum PI 184764-1, PI 184764-2, S. berthaultil PI 473331-1, S. demissum PI 161366-1 had high resistancy to two races of P. infestans tested. The clones in a same PI-number of a same species have different degree of resistancy.
The fusion hybrids between S. commersonii and the haploid potato and the sexual progenies of the hybrids were studied to detect DNA marker associated with resistance to bacterial wilt by RAPD analysis. The fusion parents, somatic hybrids and F₁ progenies were screened with 190 primer, 109 polymorphic bands were found with 10 primers. Polymorphic bands, ranging from 247 to 2053 bp products, were correspounding to an average of 11 DNA bands per primer.
The RAPD markers 243bp, 964bp of UBC #431,453bp of UBC #584, 418bp of UBC #587, 627bp of UBC #590 seems to be related to the bacterial wilt resistancy. RAPD analysis was a useful tool in determinding genetic variation and in determinding the genetic relationships. RAPD technology will provide a new alternative for detecting DNA fragments associated with useful genetic markers.

목차 (Table of Contents)

• I. 서론 = 1
• II. 연구사 = 4
• III. 재료 및 방법
• 1. 대상식물 = 9
• 2. 역병진정저항성 검정에 사용된 균주 = 12
• 3. 시약재료 = 13
• 4. 실험방법
• 1) 식물체의 기내유지 및 생장보존 = 14
• 2) 실험실 내에서의 진정저항성 검정 = 14
• 3) DNA 추출 = 17
• 4) Polymerase Chin Reaction (PCR) = l9
• 5) 전기영동 = 19
• IV. 결과 및 고찰
• 1. 역병진정저항성 검정 = 20
• 2. RAPD 분석 = 29
• V. 요약 = 44
• VI. 참고 문헌 = 46
• Appencdix = 58