형광 염색체 In Situ Suppression Hybridization의 임상적 응용에 관한 연구 = The Study for Clinical Significance of Fluorescent In Situ Suppression Hybridization

다국어 초록 (Multilingual Abstract)

A method for chromosome specific staining and its use in clinical cytogenetics is evaluated. Non isotopic fluorescent in situ hybridization provides a fast method for detection of specific nucleic acid sequences. We have used the single color fluorescent in situ hybridization with a α-satellite probe, unique sequence, microdissected probe to investigate the feasibility of simultaneous assessment of numerical and structural chromosome aberrations. When we used the α-satellits probe, Under condition of low stringency, cross hybridization with other subpopulations of the alpohoid family occurs in the whole chromosome and numerous hybridization sites are detected over metaphase. But we could findout the 3 signals in interphase chromosome of Down syndrome we could detect the signals which have been suspicted a deletion point with the unique sequence probe. We could delineate the X-Y translocated region by a microdisscted Y probe. From the above results, it can be a novel method for detection of cytogenetic abnormalities in the clinical cytogenetic field.