저출력레이저 조사가 백서의 좌골신경 재생에 미치는 영향에 관한 연구 = A STUDY ON THE EFFECTS OF LOW-POWER LASER IRRADIATION ON THE SCIATIC NERVE REGENERATION IN RATS

다국어 초록 (Multilingual Abstract)

This study was performed to investigate the effects of low-power laser (Gallium-Arsenide diode and Helium-Neon gas) application on the regeneration of experimentally cut sciatic nerve in rats. The right sciatic nerve was cut and immediately sutured by combined suture through epineurium and perineurium under microscopic field. Suture material used were 8-0 coated Vicryl (polyglactin 910) to nerve and 6-0 Mersilk to muscle and skin.
The subjects were classified into control or experimental group by without or with application of laser and experimental group was subdivided by amount of application time. The subjects were sacrified for tissue specimen at the period of postoperative 1, 2. 4 and 6 weeks, respectively, and the specimens were stained with Hematoxilin and Eosin for general findings and with Toluidine blue for nerve cells and mast cell.
The obtained results were as follows
1. In control group the regeneration were slowly and slightly progressed when compared with experimental groups. As a result, mild nerve degeneration and surrounding tissue inflammation were still observed
at postoperative 6-week in control group but in experimental groups, early regenerative changes in Schwann cell and mild fibrosis in perineural granulation tissue were shown at postoperative 4-week.
2. In postoperative findings, degeneration of axon were more severe than that of Schwann cell in both groups. But in experimental groups, degeneration of endoneurium and perineurium were rapidly decreased and mast cells were much more observed.
3. Laser irradiation on sciatic nerve regeneration was efficient, but the difference by the amount of irradiation time in two experimental groups was not distinct.