Salting-out extraction of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium based on ethanol/sodium carbonate system

다국어 초록 (Multilingual Abstract)

Background: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phasesystem recently. So far as we know, few reports involved in extracting ginsenosides with SOE because ofthe lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOEmethod for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefoliumwas established in this article.
Methods: The SOE system comprising ethanol and sodium carbonate was selected to extract ginsenosidesfrom the enzymatic hydrolysates of Panax quinquefolium, and HPLC was applied to analyze theginsenosides.
Results: The optimized extraction conditions were as follows: the aqueous two-phase extraction systemcomprising ethanol, sodium carbonate, ethanol concentration of 41.51%, and the mass percent of sodiumcarbonate of 7.9% in the extraction system under the experimental condition. Extraction time had minorinfluence on extraction efficiency of ginsenosides. The results also showed that the extraction efficienciesof three ginsenosides were all more than 90.0% only in a single step.
Conclusion: The proposed method had been successfully applied to determine ginsenosides in enzymatichydrolysate and demonstrated as a powerful technique for separating and purifying ginsenosides incomplex samples.

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