Ca2+ Signaling Induced by Sphingosine 1-Phosphate and Lysophosphatidic Acid in Mouse B Cells

다국어 초록 (Multilingual Abstract)

Lysophospholipids (LPLs) such as lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are chemotactic for lymphocytes, and increases of in cytosolic [Ca2+] signal the regulation of lymphocyte activation and migration. Here, the authors investigated the effects of LPA and S1P on [Ca2+]c in mouse B cell lines (WEHI-231 and Bal-17) and primary B cells isolated from mouse spleen and bone mar-row, and focused on the modulation of store-operated Ca2+ entry (SOCE) by LPLs. In Bal-17 (a mature B cell line) both LPA and S1P induced a transient [Ca2+]c increase via a phospholipase C pathway. In addition, pretreatment with LPLs was found to augment thapsigargin-induced SOCE in Bal-17 cells. However, in WEHI-231 (an immature B cell line) LPLs had no significant effect on [Ca2+]c or SOCE. Furthermore, in freshly isolated splenic B cells (SBCs) and bone marrow B cells (BMBCs), LPLs induced only a small increase in [Ca2+]c. Interestingly, however, pretreatment with LPLs markedly increased SOCE in primary B cells, and this augmentation was more prominent in BMBCs than SBCs. The unidirectional influx of Ca2+ was measured using Ba2+ as a surrogate ion. Similarly, Ba2+ influx was also found to be markedly increased by LPLs in SBCs and BMBCs. Summarizing, LPLs were found to strongly aug-ment SOCE-mediated Ca2+-signaling in mouse B cells. However, unlike the mature Bal-17 cell line, PLC-depen-dent Ca2+ release was insignificant in primary B cells and inWEHI-231.

참고문헌 (Reference)

1 Payne, S.G., "Sphingosine-1-phosphate:dual messenger functions" 531 : 54-57, 2002

2 Spiegel, S., "Sphingosine-1-phosphate:an enigmatic signalling lipid" 4 : 397-407, 2003

3 Itagaki, K., "Sphingosine 1-phosphate,a diffusible calcium influx factor mediating store-operated calcium entry" 278 : 27540-27547, 2003

4 Kunisawa, J., "Sphingosine 1-phosphate regulates peritoneal B-cell trafficking for subsequent intestinal IgA production" 109 : 3749-3756, 2007

5 Rosen, H., "Sphingosine 1-phosphate receptor signaling" 78 : 743-768, 2009

6 Cinamon, G., "Sphingosine 1-phosphate receptor 1 promotes B cell localization in the splenic marginal zone" 5 : 713-720, 2004

7 Aoki, J., "Serum lysophosphatidic acid is produced through diverse phospholipase pathways" 277 : 48737-48744, 2002

8 Zeng, W., "STIM1 gates TRPC channels,but not Orai1,by electrostatic interaction" 32 : 439-448, 2008

9 Goetzl, E.J., "Regulation of immunity by lysosphingolipids and their G protein-coupled receptors" 114 : 1531-1537, 2004

10 Kurosaki, T., "Regulation of B cell fates by BCR signaling components" 14 : 341-347, 2002

11 Graler, M.H., "Lysophospholipids and their G protein-coupled receptors in inflammation and immunity" 1582 : 168-174, 2002

12 Wang, L., "Lysophospholipids and chemokines activate distinct signal transduction pathways in T helper 1 and T helper 2 cells" 16 : 991-1000, 2004

13 Meyer Zu Heringdorf, D., "Lysophospholipid receptors:signalling,pharmacology and regulation by lysophospholipid metabolism" 1768 : 923-940, 2007

14 Meyer Zu Heringdorf, D., "Lysophospholipid receptor-dependent and-independent calcium signaling" 92 : 937-948, 2004

15 Noguchi, K., "Lysophosphatidic acid(LPA)and its receptors" 9 : 15-23, 2009

16 Itagaki, K., "Lysophosphatidic acid triggers calcium entry through a non-store-operated pathway in human neutrophils" 77 : 181-189, 2005

17 Yang, L., "Lysophosphatidic acid opens a Ca(++)channel in human erythrocytes" 95 : 2420-2425, 2000

18 Matloubian, M., "Lymphocyte egress from thymus and peripheral lymphoid organs is dependent on S1P receptor 1" 427 : 355-360, 2004

19 King, L.B., "Immunobiology of the immature B cell:plasticity in the B-cell antigen receptor-induced response fine tunes negative selection" 176 : 86-104, 2000

20 McIntyre, T.M., "Identification of an intracellular receptor for lysophosphatidic acid(LPA):LPA is a transcellular PPARgamma agonist" 100 : 131-136, 2003

21 Rosskopf, D., "Growth factorlike action of lysophosphatidic acid on human B lymphoblasts" 274 : C1573-C1582, 1998

22 Ralph, P., "Functional subsets of murine and human B lymphocyte cell lines" 48 : 107-121, 1979

23 Schwab, S.R., "Finding a way out:lymphocyte egress from lymphoid organs" 8 : 1295-1301, 2007

24 Yoo, H.Y., "Facilitation of Ca2+-activated K+ channels(IKCa1)by mibefradil in B lymphocytes" 456 : 549-560, 2008

25 Rosen, H., "Egress:a receptorregulated step in lymphocyte trafficking" 195 : 160-177, 2003

26 Harnett, M.M., "Differential signalling during B-cell maturation" 98 : 33-44, 2005

27 Roedding, A.S., "Characterization of the transient receptor potential channels mediating lysophosphatidic acid-stimulated calcium mobilization in B lymphoblasts" 80 : 89-97, 2006

28 Abbas, A.K., "Cellular and Molecular Immunology" 2006

29 Feske, S., "Calcium signalling in lymphocyte activation and disease" 7 : 690-702, 2007

30 Scharenberg, A.M., "Calcium signalling and cell-fate choice in B cells" 7 : 778-789, 2007

31 Lis, A., "CRACM1,CRACM2,and CRACM3 are store-operated Ca2+ channels with distinct functional properties" 17 : 794-800, 2007

32 Lambeau, G., "Biochemistry and physiology of mammalian secreted phospholipases A2" 77 : 495-520, 2008

33 Igarashi, H., "B cell Ag receptor mediates different types of signals in the protein kinase activity between immature B cell and mature B cell" 153 : 2381-2393, 1994

34 Clair, T., "Autotaxin hydrolyzes sphingosylphosphorylcholine to produce the regulator of migration,sphingosine-1-phosphate" 63 : 5446-5453, 2003

35 Takabe, K., ""Inside-out"signaling of sphingosine-1-phosphate:therapeutic targets" 60 : 181-195, 2008