Additive Antioxidant Capacity of Vitamin C and Tocopherols in Combination

다국어 초록 (Multilingual Abstract)

Vitamin C (ascorbic acid) and vitamin E(tocopherols) are water-soluble and fat-soluble essentialnutrients, respectively, present ubiquitously in cellularmembranes and plasma through dietary intake of fruits andvegetables. These vitamins act as antioxidants by virtue oftheir free hydroxyl groups. To quantitatively assess thevitamin C-tocopherol interaction in generating totalantioxidant capacity, various binary mixtures of vitamin Cand tocopherols (α, β, δ, and γ) were titrated with ABTSradicals. In this assay, the antioxidant capacity of tocopherolsincreased as follows: β-tocopherol<α-tocopherol<δ-tocopherol<γ-tocopherol. As the total concentration ofmixed antioxidants vitamin C and tocopherols increased,the total antioxidant capacity increased linearly. The sumof the individual antioxidant capacities of vitamin C andtocopherol in the assay was very close to the totalantioxidant capacity of the corresponding combination oftwo compounds. Using this assay system, and in theconcentration ranges applied, vitamin C and tocopherols incombination displayed additive interaction.

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