단삼이 활성산소에 의하여 손상된 배양 해마신경세포에 미치는 영향 = Effect of Salviae Multorrhizae Radix on The Cultured Mouse Hippocampal Neurons Damaged by Reactive Oxygen Species

다국어 초록 (Multilingual Abstract)

In order to evaluate the cytotoxic effect of reactive oxygen species(ROS), the cell viability was measured by MTT assay after cultured mouse hippocampal neurons were treated with various concentrations of xanthine oxidase(XO) and hypoxanthine (HX) for 5 hours. And also, the protective effect of Salviae Mutiorrhizae Radix(SMR) on XO/HX-induced neurotoxicity was examined in these cultures. XO/HX significantly decreased cell viability in dose. and time dependent manners when cultured mouse hippocampal neurons were treated with 5 ~ 40 mU/ml XO for 5 hours. In the protective effect of SMR, SMR increased cell viability dose-dependently after cultured mouse hippocampal neurons were preincubated with 3O ~ 12O ug/ml SM R for 2 hours. From these results, it is suggested that XO/HX is toxic on cultured mouse hippocampal neurons, and herbe medicine such as SMR is very effective in blocking the cytotoxicity induced by ROS.