역상 액체 크로마토그래피에 의한 금속-1-phenyl-3-methyl-4-(2-hydroxy-5-nitrophenylazo)-5-pyrazolone 킬레이트의 분리 및 용리거동에 관한 연구 = Separation and Elution Behavior of Some Metal-1-phenyl-3-methyl-4-(2-hydroxy-5-nitrophenylazo)-5-pyrazolone Chelates by Reverse Phase Liquid Chromatography

다국어 초록 (Multilingual Abstract)

1-Phenyl-3-methyl-4-(2-hydroxy-5-nitrophenylazo)-5-pyrazolone was synthesized, which was reacted with Ni(Ⅱ), Cu(Ⅱ), Co(Ⅲ) and Cr(Ⅲ) to form the chelate compounds. The liquid chromatographic behaviors of these metal-1-phenyl-3-methyl-4-(2-hydroxy-5-nitrophenylazo)-5-pyrazolone chelates have been stuied by the Reverse Phase High Performance Liquid Chromatography.
These four kinds of chelates were successfully separated on NOVA-PAK C?? column using methanol/water(63/37) mixture as a mobile phase. It was found that the chelates were eluted properly in an acceptable range of the capacity factor value (0≤log k＇≤1). The dependence of the capacity factor (log k＇) on the volume fraction of water in the binary mobile phase as well as on the column temperature and the enthalpy change(??H) showed a good linearity. Also, there was a good linear dependence of the capacity factor (log k＇) on the liquid-liquid extraction distribution ratio(D??) in methanol-water/n-pentadecane extraction system by the batch method. It suggested that the retention of the chelates in the reverse phase liquid chromatographic system was largely due to the hydrophobic effect.