Preventive Effect of Thiacremonone on the Hepatocarcinogenesis Initiated by N-Nitrosodiethylamine in Rats

다국어 초록 (Multilingual Abstract)

The present study was carried out to examine the chemopreventive effects of thiacremonone, which is found in heated garlic, on the formation of the preneoplastic foci and glutathione-dependent detoxifying enzyme activities in rat hepatocarcinogenesis. F344 rats were given an intraperitoneal injection of N-nitrosodiethylamine (DEN) at a dose of 200 mg/kg body weight and were then administered a gavage of thiacremonone 2 weeks after initiating the DEN treatment. All animal were subjected to a 2/3 partial hepatectomy at 3 weeks after DEN initiation. The numbers and the placental glutathione S-transferase positive (GSTP +) foci in the thiacremonone-treated groups decreased significantly compared with those in the DEN alone group.
Thiobarbituric acid reactive substances content in the thiacremonone treated group (10 mg/kg) decreased significantly compared with that in the DEN alone. These results suggest that thiacremonone may have potential chemopreventive effects on rat hepatocarcinogenesis induced by DEN.

참고문헌 (Reference)

1 권오찬, "고온고압처리에 따른 마늘(Allium sativum L.)의 이화학적 특성" 한국식품과학회 38 (38): 331-336, 2006

2 Agarwal KC, "Therapeutic actions of garlic constituents" 16 : 111-124, 1996

3 Eustis SL, "The sequential development of cancer: A morphological perspective" 49 : 267-281, 1989

4 Oshima M, "Suppression of intestinal polyposis in Apc δ716 knockout mice by inhibition of cyclooxygenase 2 (COX-2)" 87 : 803-809, 1996

5 Moore MA, "Single GST-P positive liver cells--Putative initiated hepatocytes" 8 : 483-486, 1987

6 Chun KS, "Signal transduction pathways regulating cyclooxygenase-2 expression: Potential molecular targets for chemoprevention" 68 : 1089-1100, 2004

7 Schaffer P, "Should the general population be screened for breast cancer?" 26 : 750-754, 1997

8 Tatematsu M, "Relative merits of immunohistochemical demonstrations of placental, A, B, and C forms of glutathione S-transferase and histochemical demonstration of γ-glutamyl transferase as markers of altered foci during liver carcinogenesis in rats" 6 : 1621-1626, 1985

9 Ito N, "Rapid bioassay methods for carcinogens and modifiers of hepatocarcinogenesis" 19 : 385-415, 1989

10 Kim DJ, "Promotion potential of tamoxifen on hepatocarcinogenesis in female SD or F344 rats initiated with diethylnitrosamine" 104 : 13-19, 1996

11 Kelloff GJ, "Progress in cancer chemoprevention: Development of diet-derived chemopreventive agents" 130 : 467S-471S, 2000

12 Kitano M, "Possible tumor development from double positive foci for TGF-α and GST-P observed in early stages on rat hepatocarcinogenesis" 97 : 478-483, 2006

13 Maronpot RR, "National Toxicology Program nomenclature for hepatoproliferative lesions of rats" 14 : 263-273, 1986

14 National Cancer Center in Korea, "NCCI Sheet: Cancer statistics"

15 Ogawa K, "Molecular pathology of early stage chemically induced hepatocarcinogenesis" 59 : 605-622, 2009

16 Surh Y, "Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances" 428 : 305-327, 1999

17 Park KA, "Modification of hepatic microsomal cytovhrome P450 2E1 enzyme by garlic powder in rat hepatocarcinogenesis" 30 : 73-79, 1997

18 Buege JA, "Microsomal lipid peroxidation" 52 : 302-310, 1978

19 Moore MA, "Marriage of a medium-term liver model to surrogate markers- -A practical approach for risk and benefit assessment" 27 : 237-242, 1999

20 Heon Sang Jeong, "Isolation and Identification of an Antioxidant Substance from Heated Garlic (Allium sativum L.)" 한국식품과학회 16 (16): 963-966, 2007

21 Haber-Mignard D, "Inhibition of aflatoxin B1- and N-nitrosodiethylamine-induced liver preneoplastic foci in rats fed naturally occurring allyl sulfides" 25 : 61-70, 1996

22 Endoh T, "Inhibition by acetylsalicylic acid, a cyclo-oxygenase inhibitor, and p-bromophenacylbromide, a phospholipase A2 inhibitor, of both cirrhosis and enzyme-altered nodules caused by a choline-deficient, L-amino acid-defined diet in rats" 17 : 467-475, 1996

23 Sherman M, "Hepatocellular carcinoma: Epidemiology, risk factors, and screening" 25 : 143-154, 2005

24 Jemal A, "Global cancer statistics" 61 : 69-90, 2011

25 Milner JA, "Garlic: Its anticarcinogenic and antitumorigenic properties" 54 : S82-S86, 1996

26 Pedraza-Chaverri J, "Garlic prevents hypertension induced by chronic inhibition of nitric oxide synthesis" 62 : PL71-PL77, 1998

27 Greenwald P, "From carcinogenesis to clinical interventions for cancer prevention" 166 : 37-45, 2001

28 Bae SH, "Expression of cyclooxygenase-2 (COX-2) in hepatocellular carcinoma and growth inhibition of hepatoma cell lines by a COX-2 inhibitor, NS-398" 7 : 1410-1418, 2001

29 Takada N, "Enhancement by organosulfur compounds from garlic and onions of diethylnitrosamine-induced glutathione S-transferase positive foci in the rat liver" 54 : 2895-2899, 1994

30 Jang JJ, "Effects of allyl sulfide, germanium, and NaCl on the development of glutathion S-transferase P-positive rat hepatic foci initiated by diethylnitrosamine" 9 : 273-275, 1989

31 Hadjiolov D, "Effect of diallyl sulfide on aristolochic acid-induced forestomach carcinogenesis in rats" 14 : 407-410, 1993

32 Wargovich MJ, "Diallyl sulfide. A naturally occurring thioether that inhibits carcinogen-induced nuclear damage to colon epithelial cells in vivo" 143 : 127-129, 1985

33 Cheng JY, "Detection of human papillomavirus DNA in colorectal carcinomas by polymerase chain reaction" 37 : 87-90, 1995

34 Cheng JY, "Detection of human papillomavirus DNA in colorectal adenomas" 130 : 73-76, 1995

35 Ogiso T, "Comparative effects of carcinogens on the induction of placental glutathione Stransferase- positive liver nodules in a short-term assay and of hepatocellular carcinomas in a long-term assay" 13 : 257-265, 1985

36 Kim DJ, "Chemoprevention of colon cancer by Korean food plant components" 523 : 99-107, 2003

37 Marquez-Rosado L, "Celecoxib, a cyclooxygenase-2 inhibitor, prevents induction of liver preneoplastic lesions in rats" 43 : 653-660, 2005

38 Sporn MB, "Approaches to prevention of epithelial cancer during the preneoplastic period" 36 : 2699-2702, 1976

39 Ito N, "A medium-term rat liver bioassay for rapid in vivo detection of carcinogenic potential of chemicals" 94 : 3-8, 2003