경기도내 수계시설에서 분리된 레지오넬라균의 분포현황 및 Legionella pneumophila serogroup 1의 유전학적 다양성 연구 = Distribution of Legionella species from water systems and genetic diversity of L. pneumophila serogroup 1 in Gyeonggi-do

국문 초록 (Abstract)

레지오넬라증은 인공수계환경이 레지오넬라균에 오염되었을 때 에어로졸의 전파에 의하여 집단 발생되는 심각한 폐렴과 높은 치명률을 일으키는 호흡기질환이다. 본 연구에서는 경기도내 수계시설로부터 147주의 레지오넬라균을 검출하였고, 시설별, 수계환경별 분포현황을 조사하였다. 분리한 레지오넬라균 중에서 Legionella pneumophila의 검출률은 85.7%로 높게 나타났으며, 혈청군을 분석한 결과 L. pneumophila serogroup (sg) 1이 주로 검출되었다. Non-L. pneumophila 중에서는 L. wadsworthii이 가장 많이 검출되었다. L. pneumophila sg 1의 유전학적 다양성을 분석하기 위해 pulsed field gel electrophoresis (PFGE)와 sequence-based typing (SBT) 방법을 사용하여 비교 분석하였다. 32주에 대해 PFGE 분석 결과 22개의 PFGE pattern을 보였고, SBT 분석 결과 9개의 유형으로 나누어졌다. PFGE와 SBT 분석에서 모두 크게 3개 group으로 분류되어 매우 유사한 결과를 나타냈다. SBT 유형 중 ST1이 가장 우점하였고, 2개의 새로운 유형도 찾아낼 수 있었다. SBT는 데이터베이스가 잘 구축되어 있어 균주 간의 유전학적 유형분석을 쉽고, 간단하게 할 수 있기 때문에 레지오넬라증의 분자역학적 연구에 유용한 방법임을 확인할 수 있었다.

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