Korean Red Ginseng extract ameliorates melanogenesis in humans and induces antiphotoaging effects in ultraviolet B – irradiated hairless mice

다국어 초록 (Multilingual Abstract)

Background: Panax ginseng is a marvelous herbal remedy for all ailments of body. That may be why it iscalled Panax, which means “cure for all”. Melanin is a pigment that gives color to our skin; however,increased melanin production can lead to tumor formation. Human exposure to ultraviolet B radiationhas increased extensively owing to the increased sunlight due to global warming. Consequently, aphenomenon called photoaging has been observed for all skin colors and types. As a result of thisphenomenon, a set of enzymes called matrix metalloproteinases, which serve as degradation enzymesfor extracellular matrix proteins, mainly collagen, is increased, causing depletion of collagen andresulting in early wrinkle formation.
Methods: Therefore, in our study, we used the murine melanoma cell line B16/F10 to study the inhibitionof melanogenesis by Korean Red Ginseng (KRG) extract in vitro and HRM-2 hairless mice exposed toartificial ultraviolet B to examine the efficacy of KRG in vivo. We prepared a 3% red ginseng extract creamand evaluated its effects on human skin.
Results: Our results demonstrated that KRG induced potent suppression of tyrosinase activity andmelanin production in B16/F10 cells; moreover, it reduced the transcription and translation of componentsinvolved in the melanin production pathway. In the in vivo experiments, KRG potently suppressedthe expression of matrix metalloproteinases, reduced wrinkle formation, and inhibited collagen degradation.
On human skin, ginseng cream increased skin resilience and skin moisture and enhanced skintone.

참고문헌 (Reference)

1 김의검, "신공법에 의한 흑삼의 제조 및 항암활성" 대한본초학회 23 (23): 85-92, 2008

2 Wu M, "c-Kit triggers dual phosphorylations, which couple activation and degradation of the essential melanocyte factor Mi" 14 : 301-312, 2000

3 Brenner M, "The protective role of melanin against UV damage in human skin" 84 : 539-549, 2008

4 Gillbro JM, "The melanogenesis and mechanisms of skin-lightning agents–existing and new approaches" 33 : 210-221, 2011

5 Yokota T, "The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation" 11 : 355-361, 1998

6 Helfrich YR, "Overview of skin aging and photoaging" 20 : 177-183, 2008

7 Roméro-Graillet C, "Nitric oxide produced by ultraviolet-irradiated keratinocytes stimulates melanogenesis" 99 : 635-, 1997

8 Masamoto Y, "Mushroom tyrosinase inhibitory activity of esculetin isolated from seeds of Euphorbia lathyris L" 67 : 631-634, 2003

9 Tam I, "Melanocytes-immunocompetent pigmented cells" 24 : 188-, 2007

10 Mizoguchi M, "Melanocyte development : with a message of encouragement to young women scientists" 17 : 533-544, 2004

11 Fisher GJ, "Mechanisms of photoaging and chronological skin aging" 138 : 1462-1470, 2002

12 Quan T, "Matrix-degrading metalloproteinases in photoaging" 14 : 20-24, 2009

13 Jablonska-Trypuc A, "Matrix metalloproteinases(MMPs), the main extracellular matrix(ECM)enzymes in collagen degradation, as a target for anticancer drugs" 31 : 177-183, 2016

14 Brennan M, "Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin" 78 : 43-48, 2003

15 Chang JY, "Masson trichrome stain helps differentiate myofibroma from smooth muscle lesions in the head and neck region" 107 : 767-773, 2008

16 Cardiff RD, "Manual hematoxylin and eosin staining of mouse tissue sections" 2014 : 655-658, 2014

17 김혜림, "Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Melanogenesis in B16F10 Mouse Melanoma Cells" 한국분자세포생물학회 38 (38): 163-170, 2015

18 Margaret Song, "Korean Red Ginseng Powder in the Treatment of Melasma: An Uncontrolled Observational Study" 고려인삼학회 35 (35): 170-175, 2011

19 Cabanes J, "Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor of catecholase activity of tyrosinase" 46 : 982-985, 1994

20 Tobin DJ, "Introduction to skin aging" 26 : 37-46, 2017

21 El-Domyati M, "Intrinsic aging vs. photoaging : a comparative histopathological, immunohistochemical, and ultrastructural study of skin" 11 : 398-405, 2002

22 Hu YH, "Inhibitory kinetics of chlorocinnamic acids on mushroom tyrosinase" 117 : 142-146, 2014

23 Kim DS, "Inhibitory effects of 4-n-butylresorcinol on tyrosinase activity and melanin synthesis" 28 : 2216-2219, 2005

24 Englaro W, "Inhibition of the mitogen-activated protein kinase pathway triggers B16 melanoma cell differentiation" 273 : 9966-9970, 1998

25 Lee HJ, "Hesperidin, a popular antioxidant inhibits melanogenesis via erk1/2 mediated MITF degradation" 16 : 18384-18395, 2015

26 Ohguchi K, "Gnetol as a potent tyrosinase inhibitor from genus Gnetum" 67 : 663-665, 2003

27 Christensen LP, "Ginsenosides chemistry, biosynthesis, analysis, and potential health effects" 55 : 1-99, 2009

28 김보민, "Ginsenoside Rg3 Induces Apoptosis of Human Breast Cancer (MDA-MB-231) Cells" 대한암예방학회 18 (18): 177-185, 2013

29 Goldstein B., "Ginseng: its history, dispersion, and folk tradition" 3 : 223-234, 1975

30 Dai D, "Ginseng on cancer : potential role in modulating inflammation-mediated angiogenesis" 45 : 13-22, 2017

31 Chevallier A, "Encyclopedia of medicinal plants. St Leonards" Dorling kindersley pty limited 1996

32 Evelyn Saba, "Black ginseng-enriched Chong-Myung-Tang extracts improve spatial learning behavior in rats and elicit anti-inflammatory effects in vitro" 고려인삼학회 41 (41): 151-158, 2017

33 Evelyn Saba, "Black ginseng extract ameliorates hypercholesterolemia in rats" 고려인삼학회 40 (40): 160-168, 2016

34 Bissett DL, "An animal model of solar-aged skin : histological, physical, and visible changes in UV-irradiated hairless mouse skin" 46 : 367-378, 1987

35 Evelyn Saba, "Alleviation of diabetic complications by ginsenoside Rg3-enriched red ginseng extract in western diet-fed LDLe/e mice" 고려인삼학회 42 (42): 352-355, 2018

36 Evelyn Saba, "Acetyl Eburicoic Acid from Laetiporus sulphureus var. miniatus Suppresses Inflammation in Murine Macrophage RAW 264.7 Cells" 한국균학회 43 (43): 131-136, 2015

37 Wakabayashi Y, "Abrogating effect of N-linked carbohydrate modifiers on the stem cell factor and endothelin-1-stimulated epidermal pigmentation in human epidermal equivalents" 69 : 215-228, 2013

38 Kim DS, "A new 2-imino-1, 3-thiazoline derivative, KHG22394, inhibits melanin synthesis in mouse B16 melanoma cells" 30 : 180-183, 2007