글루코즈 - 6 - 인산 가수분해 효소의 중간체 표지 = Steady -State labeling of Hepatic Gucose - 6 - Phosphotase

다국어 초록 (Multilingual Abstract)

Glucose-6-phosphatase has been known as a multicomponent system and glucose-6-phosphatase was labeledd by incubating with [^(32)P]glucose-6-phosphate to be saturation concentration. Microsomal pellet was obtained by the method of differential ultracentrifugation and distrupted with sonication. To this microsomal protein mixture, [^(32)P]glucose-6-phosphate was added and incubated. Then the mixture was seperated with 10∼15% gradient PAGE and the gel was dried and exposed on the X-ray film. As a result, 60 kd band was obtained and this was considered as a glucose-6-phosphatase. The same result was obtained when [^(32)P]mannose-6-phosphate and [^(32)P]galactose-6-phosphate were used as substrates. 20% trichloro acetic acid. 8 M urea, and 10% SDS were used as a quenching system respectively and 60 kd band was still unchanged.