South Korea's animal welfare regulations have improved, promoting aviary, floor, and barn systems for laying hens. However, research on feeds for floor-raised hens remains to be limited due to low profitability in the poultry feed sector. Furthermore,...
South Korea's animal welfare regulations have improved, promoting aviary, floor, and barn systems for laying hens. However, research on feeds for floor-raised hens remains to be limited due to low profitability in the poultry feed sector. Furthermore, the transition to animal welfare-certified cage-free systems, which are more susceptible to bacterial diseases compared to cage housing, highlights the necessity for cost-effective dietary interventions to improve productivity and mitigate stress. This study investigated the effects of Glucose Oxidase (GOX) and Enterosure (probiotics) on productivity, egg quality, and stress reduction in laying hens under commercial farming conditions. A total of 60,488 Lohmann Brown-Lite laying hens were allocated to one of three dietary treatments: a basal diet (CON), CON supplemented with 0.03% GOX (T1), and CON supplemented with 0.02% Enterosure (T2). Key indicators, including feed conversion ratio (FCR), egg mass, egg quality parameters, and corticosterone levels, were monitored during six weeks. The results showed no significant differences in productivity such as FCR, egg mass, or egg production rate, among the treatment groups. However, T1 significantly improved yolk color (p < 0.05), likely due to the antioxidant effects of GOX enhancing carotenoid stability. Corticosterone levels declined across all groups, with T1 showing the largest reduction (34.6%), indicative of potential stress-mitigating properties despite a lack of statistical significance. Other egg quality parameters, such as shell thickness and Haugh unit, showed no significant differences between dietary treatments. The lack of notable improvements in productivity would be attributed to high quality basal diets or less optimal additive dosages. The findings highlight the need for further research to optimize the use of dietary additives in commercial and animal welfare-focused systems. Future trials should explore synergistic additive combinations, longer study durations, and cost-benefit analyses to maximize their practical utility in the poultry industry.