회전하는 산소전달장치가 부착된 동물세포 배양기의 조업 성능에 관한 고찰 = Performance of a Novel Bioreactor Equipped with Moving Membrane Tube-Aeration System

다국어 초록 (Multilingual Abstract)

The optimal conditions for operating a moving-aeration bioreactor were determined as 30 rpm and 150 (㎖/min) of air flow rate, which can yield ca. 7.3 (ℓ/h) of maximum mass transfer coefficient. It was also found that the agitation speed played much important role than air input rate in oxgen transfer into the medium. 2.6×10exp(6) (cells/㎖) and 0.6 (㎎/ℓ) of maximum cell density and IL-2 production were observed in batch cultivation of IL-2 producing BHK cell line. 0.53 (mM/ℓ/h) of oxygen uptake rate was also estimated. The performance of a moving-aeration bioreactor (specific growth rate and oxygen uptake rate, etc.) was superior to other culture systems, such as cell-lift and static membrane aeration bioreactors. Ii must be useful to apply this reactor to many culture processes by improving structural limitations in scaling-up the system.