The Frog Embryo Teratogenesis Assay-Xenopus(FETAX) protocol has recently been adopted as a valuable tool for evaluating the embryotoxicity of chemical toxicity and environmental contaminants in frogs. In this experiment, embryotoxicity of the Pb2+ was...
The Frog Embryo Teratogenesis Assay-Xenopus(FETAX) protocol has recently been adopted as a valuable tool for evaluating the embryotoxicity of chemical toxicity and environmental contaminants in frogs. In this experiment, embryotoxicity of the Pb2+ was determined in Korean frog, Hyla japonica using the FETAX protocol. The percentage of mortality as well as the percentage of malformed larvae was investigated by probit analysis. The 96-h LC50 of 23.9 ㎎/ℓ and EC50 of 13.8 ㎎/ℓ, a TI of 1.73 was derived; indicating that Carbofuran is to be considered a teratogenic compound.
From the above results, it reveal that Carbofuran in this experiment suppressed the development of embryos at relatively low concentration. Therefore, the Hyla japonica embryo teratogenesis assay system can be used as a useful tool to evaluate the toxicity of the pollutants in environment.