Blood-anticoagulant activities of the purified fucoidans of Laminaria religiosa, sporophylls of Undaria pinnatifida, Hijikia fusiforme and Sargassum fulvellum

국문 초록 (Abstract)

국내산 갈조류로부터 생리 기능성이 우수한 식이섬유 소재를 개발하기 위한 기초 자료를 얻 기 위해 다시마, 미역 (엽상체와 포자엽), 톳, 모자반으로부터 fucoidan의 항혈액응고 작용 특성을 조사하였다. 다시마, 미역포자엽, 톳 모자반 정제 fucoidan의 항혈액응고력을 측정한 결과 A PTT{Activated pa π ial thromboplasin time) 측정값이 다시마 , 돗 , 모자반 , 미역 포자엽 순으로 높았으며, 특히 다시마의 APTT 값이 높아 18ug/ml에서 70초로 heparine의 약 15% 활성을 나타내었다. 또한 다시마, 톳, 모자반 정제 fucoidan의 항혈액응고력은 황산기의 함량이 많을 수록 증가하였으며 일정 농도이상에서는 APTT 값이 급격히 증가하는 농도 의존성올 나타내었다.

다국어 초록 (Multilingual Abstract)

This research was done to investigate functional properties of fucoidans from manne brown algae in Korea. Blood-anticoagulant activities of purified fucoidans prepared from Laminaria religiosa, sporophylls of Undaria pinnatlfida, Hijikia fusiforme and Sargassum fulvellum Were evaluated by use of PT(Prothrombin time) and APTT(Activated partial thromboplastin timehests. All purified fucoidans had no effects on PT results, but they prolonged clotting times in APTT test. Blood-coagulant time, which was measured by APTT test, of purified fucoidans from L, religiosa, H. fusiforme, S. fulvellum and U. pinnatifida were 70, 45, 40, and 39sec, respectively. Blood-coagulation times of fucoidans appeared to increase with increase in the molar ratio of ester sulfate to fucose and galactose of fucoidans. Blood-anticoagulant activities of purified fucoidans from L. religiosa, H. fushiforme and U. pinnatifidain sharply increased with increasing their concentration, but purified fucoidan from S. fulvellum is steadily incrcased.

목차 (Table of Contents)

• ABSTRACT
• Ⅰ.Introduction
• Ⅱ.Materials and Methods
• Ⅲ.Results and Discussion
• Ⅳ.References
• 초록