幼若家兎의 長管骨 骨端成長板에 X 線照射가 미치는 影響에 關한 組織學的 硏究 = Histological Study on the Effect of X-Irradiation upon the Epiphyseal Plate of Long Bone in the Growing Rabbits

다국어 초록 (Multilingual Abstract)

The effect of X-irradiation upon the growth of long and flat bones has been of interest ever since the discovery of roentgen ray in 1895.
The roentgen ray has been applied very effectively for the diagnosis and treatment of diseases but complications such as the stunting of bone growth, fracture, radiation osteitis, decalcification, bony necrosis and very rarely malignant tumors of bone have been reported to occur in the course of roentgen ray therapy.
Since the appearance of Perthes’ report(1903) which first demonstrated gross underdevelopment of osseous structures following X-irradiation, numerous investigations have been recorded on the subject of the effect of X-irradiation on the skeletal system. From a careful review of all the literatures available, it appears that there has been a conspicuous lack of clearness about the amount of radiation doses necessary to inhibit the growth of bone, the duration of the effect and the accompanying histological changes.
The author, therefore, has undertaken this study in order to obtain the detailed histological changes of the epiphyseal plates in the growing rabbits following X-irradiation.
Experimental method:
One handred rabbits weighing 500 to 700 gm were used all experiments. These were subdivided into Group Ⅰ, Ⅱ and Ⅲ according to the method of X-irradiation to the right knee:
Group Ⅰ: A total dose of 3000 R was given in 6 increments within 3 weeks.
Group Ⅱ: A total dose of 3000 R was given in 3 increments within 3 weeks.
Group Ⅲ: A single dose of 3000 R was given at one setting.
The irradiated rabbits were sacrificed at the 1st, 2nd, 3rd, 4th, 6th, and 8th week respectively and the histological observations of the epiphyseal plates of distal femurs were made.
Major findings observed are summarized as follows:
1. The distortion of cartilage columns and the atrophy of epiphyseal plates following X-irradiation were found in each group.
2. In Group Ⅰ and Ⅱ, the damaged epiphyseal plates following X-irradiation began to regenerate at the 4th week and returned almost to normal feature at the 6th-8th week. On the other hand, in Group Ⅲ the calcified cells remained persistently at the zone of developing trabeculae of metaphysic as well as at the zone of calcifing cartilage and therefore normal ossification process of the trabeculae was disturbed and delayed.
3. Bony change at the zone of trabeculae following X-irradiation was mild in Group Ⅰ, moderate in Group Ⅱ and most severe in Group Ⅲ.
4. Regeneration process of the damaged epiphyseal plates began early in Group Ⅰ and it was most retarded in group Ⅲ.
5. In group Ⅲ, there were fatty infiltration and few cellular elements in the bone marrow cavity, and myelofibrosis of the marrow tissue appeared. Therefore, it was apparent that the regeneration process was retarded.
6. X-irradiation in divided doses causes less damage to the epiphyseal plate and accordingly regenerative process was so much faster.

목차 (Table of Contents)

• Ⅰ. 緖論
• Ⅱ. 實驗材料 및 方法
• Ⅲ. 實驗成績
• A. 長管骨骨端成長板의 肉眼的 所見
• B. 長管骨骨端成長板의 組織學的 所見
• 1. 正常對照群
• 2. 實驗群
• a) 第1群
• ㄱ) 最終照射日부터 1週後所見
• ㄴ) 最終照射日부터 2週後所見
• ㄷ) 最終照射日부터 3週後所見
• ㄹ) 最終照射日부터 4週後所見
• ㅁ) 最終照射日부터 6週後所見
• ㅂ) 最終照射日부터 8週後所見
• b) 第2群
• ㄱ) 最終照射日부터 1週後所見
• ㄴ) 最終照射日부터 2週後所見
• ㄷ) 最終照射日부터 3週後所見
• ㄹ) 最終照射日부터 4週後所見
• ㅁ) 最終照射日부터 6週後所見
• ㅂ) 最終照射日부터 8週後所見
• c) 第3群
• ㄱ) 最終照射日부터 1週後所見
• ㄴ) 最終照射日부터 2週後所見
• ㄷ) 最終照射日부터 3週後所見
• ㄹ) 最終照射日부터 4週後所見
• ㅁ) 最終照射日부터 6週後所見
• ㅂ) 最終照射日부터 8週後所見
• Ⅳ. 總括 및 考按
• Ⅴ. 結論
• 參考文獻
• 寫眞附圖