알로에 겔의 in vitro 생리기능 특성에 미치는 삼투탈수공정의 영향 = Effect of DIS Process on in vitro Physiological Functionality of Aloe vera Gel

다국어 초록 (Multilingual Abstract)

In vitro physiological functions such as jack bean (Canavalia ensiformis) urease inhibitory activity and retarding
effect of glucose/bile acid of Aloe vera gel concentrated by the optimized DIS (Dewatering Impregnation & Soaking)
process conditions were examined. Urease inhibitory activity of DIS aloes ranged from 84.6 to 94.4%, which
was similar to or higher than 86.3% of fresh aloe. Also, urease inhibitory activity of DIS aloes was maintained at
initial levels after heat treatment (90oC, 10 min.) and drying treatment (freeze or hot air drying). Urease inhibition
pattern from Lineweaver-Burk plot indicated general non-competitive inhibition, and inhibition constants (KIE and
KIES) of DIS aloes were 41-149 and 87-163 μL/mL, respectively. DIS(glucose) and DIS(polyethylene glycol) exhibited
the highest retarding effect of glucose and bile acid. Their retarding effects were about 1.6 and 1.8 folds higher
than that of fresh aloe after 0.5 and 1 hr of the dialysis, respectively. Conclusively, the above in vitro physiological
functions of Aloe vera gel concentrated by DIS process suggested that aloe products treated with DIS would have
the potential benefits for protection against Helicobacter pylori and reduction of blood glucose and cholesterol levels.

다국어 초록 (Multilingual Abstract)

In vitro physiological functions such as jack bean (Canavalia ensiformis) urease inhibitory activity and retarding
effect of glucose/bile acid of Aloe vera gel concentrated by the optimized DIS (Dewatering Impregnation & Soaking)
process conditions were examined. Urease inhibitory activity of DIS aloes ranged from 84.6 to 94.4%, which
was similar to or higher than 86.3% of fresh aloe. Also, urease inhibitory activity of DIS aloes was maintained at
initial levels after heat treatment (90oC, 10 min.) and drying treatment (freeze or hot air drying). Urease inhibition
pattern from Lineweaver-Burk plot indicated general non-competitive inhibition, and inhibition constants (KIE and
KIES) of DIS aloes were 41-149 and 87-163 μL/mL, respectively. DIS(glucose) and DIS(polyethylene glycol) exhibited
the highest retarding effect of glucose and bile acid. Their retarding effects were about 1.6 and 1.8 folds higher
than that of fresh aloe after 0.5 and 1 hr of the dialysis, respectively. Conclusively, the above in vitro physiological
functions of Aloe vera gel concentrated by DIS process suggested that aloe products treated with DIS would have
the potential benefits for protection against Helicobacter pylori and reduction of blood glucose and cholesterol levels.

참고문헌 (Reference)

1 이인선, "호장근에 의한 Helicobacter pylori의 생육 저해" 한국식품과학회 35 (35): 1182-1187, 2003

2 김성아, "삼투탈수 알로에 건조제품의 구조적 및 물리화학적 특성" 한국산업식품공학회 13 (13): 24-31, 2009

3 김성아, "삼투탈수 공정에 의한 Aloe vera gel의 농축과 이의 동력학적 특성" 한국산업식품공학회 12 (12): 198-208, 2008

4 김성아, "삼투용질종류, 압력 및 교반공정이 Aloe vera gel의 삼투탈수공정에 미치는 효과" 한국산업식품공학회 12 (12): 275-283, 2008

5 Miller GL, "Use of dinitrosalicylic acid reagent for determination of reducing sugars" 31 : 426-428, 1959

6 Marshall BJ, "Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration" 323 : 1311-1315, 1984

7 Jenkins DJA, "Unabsorbable carbohydrates and diabetes: decreased post-prandial hyper-glycemia" 24 : 172-174, 1976

8 Palou E, "The use of Peleg's equation to model osmotic concentration of papaya" 12 : 965-978, 1994

9 Giudice GD, "The design of vaccines against Helicobacter pylori and their development" 19 : 523-563, 2001

10 Lee JJ, "The antimicrobial activity of medicinal plants extracts against Helicobacter pylori" 31 : 764-770, 1999

11 Grindlay D, "The aloe vera phenomena: a review of the properties and uses of the leaf parenchyma gel" 16 : 117-151, 1986

12 Matsubara S, "Suppression of Helicobacter pylori-induced gastritis by green tea extract in Mongolian ger bils" 310 : 715-719, 2003

13 Nanjundaswamy AM, "Studies of development of new categories of dehydrated product from indigenous fruits" 22 : 91-93, 1978

14 Ciurli S, "Structural properties of the nickel ions in urease:novel insight into the catalytic and inhibition mechanisms" 190-192 : 331-335, 1999

15 Henderson PJF, "Statistical analysis of enzyme kinetic data.In: Techniques in the Life Sciences: Techniques in Protein and Enzyme Biochemistry-Part II, B113" Elsevier 1-43, 1978

16 Schneeman BO, "Soluble vs. insoluble fiber-different physiological responses" 41 : 81-82, 1987

17 Lee KS, "Retarding effect of dietary fibers on the glucose and bile acid movement across a dialysis membrane in vitro" 29 : 738-746, 1996

18 Lopez G, "Relationship between physical and hydration properties of soluble and insoluble fiber of articoke" 44 : 2773-2778, 1996

19 Anderson JW, "Plant fiber. Carbohydrate and lipid metabolism" 32 : 346-363, 1979

20 Thiobault JF, "Physico-chemical properties of food plant cell walls. In: Dietary Fibres - A component of food" Springer 21-39, 1992

21 Ponting ID, "Osmotic dehydration of fruits-Recent modifications and applications" 8 : 18-20, 1973

22 Hawkes J, "Osmotic concentration of fruit slices prior to freeze dehydration" 2 : 265-284, 1978

23 McGee DJ, "Mechanisms of Helicobacter pylori infection: bacterial factors" 241 : 155-180, 1999

24 White CA, "Manual and automated spectrophotometric techniques for the detection and assay of carbohydrates and related molecules. In: Techniques in arbohydrate Metabolism,B312" Elsevier 1-64, 1981

25 Galal EE, "Kandil A, Hegazy R. 1975. Aloe vera and gastrogenic ulceration" 7 : 73-76, 1975

26 KFDA, "KFDA.2008"

27 Tabak M, "In vitro inhibition of Helicobacter pylori by extracts of thyme" 80 : 667-672, 1996

28 Blaser MJ, "Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation" 102 : 720-727, 1992

29 Femenia A, "Effects of heat treatment and dehydration on bioactive polysaccharide acemannan and cell wall polymers from Aloe barbadensis Miller" 51 : 397-405, 2003

30 Saito H, "Effects of aloe extract,Aloctin A on gastric secretion and on experimental gastriclesions in rats" 109 : 335-339, 1989

31 한성희, "Effect of heat treatment of digestion-resistant fraction from soybean on retarding of bile acid transport in vitro" 한국영양학회 3 (3): 149-155, 2009

32 Krotkiewski M, "Effect of guar gum on body-weight, hunger ratings and metabolism in obese subjects" 52 : 97-105, 1984

33 Kay RM, "Effect of citrus pectin on blood lipids and fecal steroid excretion in man" 30 : 171-175, 1977

34 Adiotomre J, "Dietary fiber: in vitro methods that anticipate nutrition and metabolic activity in humans" 52 : 128-134, 1990

35 Story JA, "Dietary fiber and lipid metabolism" 180 : 447-452, 1985

36 Baek JH, "Development of functional materials from Aloe vera gel fractions with different molecular sizes and their functionality characterization" Kangwon National University 2008

37 Trowell, H, "Definition of dietary fiber and hypotheses that it is a protective factor in certain diseases" 29 : 417-427, 1976

38 Femenia A, "Compositional features of polysaccharides from aloe vera (Aloe barbadensis Miller) plant tissues" 39 : 109-117, 1999

39 Hamman JH, "Composition and applications of Aloe vera leaf gel" 13 : 1599-1616, 2008

40 Mobley HLT, "Characterization of urease from Campylobacter pylori" 26 : 289-292, 1988

41 Jun HI, "Characterization of the pectic polysaccharides from pumpkin peel" 39 : 554-561, 2006

42 Boyd GS, "Bile acids in the rat:studies in experimental occlusion of the bile duct" 7 : 83-94, 1966

43 Sugiyama T, "Attributable risk of H. pylori in peptic ulcer disease" 46 : 307-310, 2001

44 Teradaira R, "Anti-gastriculcer effects of Aloe arborescens Mill. var. natalensis Berger" 7 : 34-36, 1993

45 Coat BC, "Aloe vera the silent healer. A modern study of Aloe vera" Bill Coats 228-294, 1979

46 Reynolds T, "Aloe vera leaf gel: a review update" 68 : 3-37, 1999

47 Juszkiewicz A, "A study of the inhibition of jack bean urease by garlic extract" 85 : 553-558, 2004