A report of 42 unrecorded bacterial species belonging to the Alphaproteobacteria in Korea

다국어 초록 (Multilingual Abstract)

As a subset study to discover indigenous prokaryotic species in Korea, a total of 42 bacterial strains assigned to the class Alphaproteobacteria were isolated from diverse environmental habitats including plant roots, ginseng soil, forest soil, marsh, mud flat, freshwater, and seawater. From the high 16S rRNA gene sequence similarity (>99.1%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 42 species have been described in Korea; therefore 4 species of 1 genera in the order Caulobacterales, 18 species of 10 genera in the order Rhizobiales, 7 species of 5 genera in the order Sphingomonadales and 13 species of 11 genera in the order Rhodobacterales within the Alphaproteobacteria are reported for alphaproteobacterial species found in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

참고문헌 (Reference)

1 Moreno, E., "The prokaryotes: a handbook on the biology of bacteria" Springer 2006

2 Fredricks, D. N., "The prokaryotes: a handbook on the biology of Bacteria" Springer 2006

3 Saitou, N., "The neighbor-joining method: a new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

4 Alsmark, C. M., "The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae" 101 : 9716-9721, 2004

5 Kimura, M., "The Neutral Theory of Molecular Evolution" Cambridge University Press 1983

6 Thompson, J. D., "The Clustal_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools" 25 : 4876-4882, 1997

7 Felsenstein, J., "PHYLIP (phylogeny inference package), version 3.6a" Department of genetics, University of Washington 2002

8 Andersson, S. G., "On the origin of mitochondria: a genomics perspective" 358 : 165-177, 2003

9 Ferla, M. P., "New rRNA gene-based phylogenies of the Alphaproteobacteria provide perspective on major groups, mitochondrial ancestry and phylogenetic instability" 8 : e83383-, 2013

10 Skorpil, P., "Molecular interactions between Rhizobium and legumes" 41 : 143-164, 2006

11 Gray, M. W., "Mitochondrial evolution" 283 : 1476-1481, 1999

12 Kersters, K., "Introduction to the Proteobacteria. In the prokaryotes: a handbook on the biology of bacteria" Springer 2006

13 Kim, O. S., "Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species" 62 : 716-721, 2012

14 Giovannoni, S. J., "Genome streamlining in a cosmopolitan oceanic bacterium" 309 : 1242-1245, 2005

15 Lee, H. J., "Flavihumibacter solisilvae sp. nov., isolated from forest soil" 64 : 2897-2901, 2014

16 Kaneko, T., "Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110" 9 : 189-197, 2002

17 Kaneko, T., "Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti" 7 : 331-338, 2000