누에에서 생산된 뱀장어 재조합 생식소자극호르몬이 암컷 뱀장어(Anguilla japonica)의 성성숙 유도에 미치는 영향 = Induction of Sexual Maturation in Female Eels Anguilla japonica by Recombinant Eel Gonadotropin Produced in Silkworm

다국어 초록 (Multilingual Abstract)

This study investigated the effects of recombinant eel gonadotropin hormone (rJeGTH) produced in silkworms, withand without a carboxyl-terminal peptide from equine chorionic gonadotropin (eCG), on the induction of sexual maturationin female eels Anguilla japonica. Experiments were conducted both in vivo and in vitro. In in vitro trials, germinalvesicle breakdown (GVBD) induction did not significantly differ between rJeFSH and rJeFSH∙eCG treatmentsand the control group. However, previous studies did find that rJeLH and rJeLH∙eCG treatments induced GVBD infemale eels. Our in vitro exploration of estradiol-17β (E2) levels in immature ovarian tissues revealed significantlyhigher E2 levels in the group treated with rJeFSH∙eCG 1 μg/mL than in the control group. In contrast, the in vivoexperiments showed no effect of recombinant hormones on the sexual maturation of feminized eels. Previous studiesand our own in vitro results have clearly shown that rJeGTH and rJeGTH∙eCG have a positive effect on the sexualmaturation of feminized eels. To develop the activity of rJeGTH in vivo, further studies should confirm circulationtime and activity of these hormones in eels’ bloodstream, modify the structure of the recombinant gene, and implementadditional glycosylation.

참고문헌 (Reference)

1 김대중, "자연산 뱀장어의 인위적인 성숙 유도에 따른 혈중 성호르몬 변동" 한국양식학회 19 (19): 267-274, 2006

2 김대중, "열목어 재조합 생식선자극호르몬(mt-rGTH)에 의한 암컷 뱀장어의 성성숙 유도" 한국발생생물학회 12 (12): 261-266, 2008

3 김대중, "극동산 뱀장어, Anguilla japonica 자성화에 미치는 estradiol-17β 유도 효과" 한국생명과학회 23 (23): 998-1003, 2013

4 Boëtius J, "Studies of ovarian growth induced by hormone injections in the European and American eel(Anguilla Anguilla L. and Anguilla rostrate LeSueur)" 3 : 183-198, 1962

5 Claire Cahoreau, "Structure–Function Relationships of Glycoprotein Hormones and Their Subunits’ Ancestors" Frontiers Media SA 6 : 1-14, 2015

6 K. YAMAMOTO, "Sexual maturation of Japanese eel and production of eel larvae in the aquarium" Springer Nature 251 (251): 220-222, 1974

7 Gregorio Molés, "Receptor Specificity and Functional Comparison of Recombinant Sea Bass (Dicentrarchus labrax) Gonadotropins (Fsh and Lh) Produced in Different Host Systems1" Oxford University Press (OUP) 84 (84): 1171-1181, 2011

8 Makito Kobayashi, "Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larvae" Elsevier BV 167 (167): 379-386, 2010

9 Dae-Jung Kim, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" Elsevier BV 233 : 8-15, 2016

10 Kazeto Y., "Ovarian steroidogenesis and the role of sex steroid hormones on ovarian growth and maturation of the Japanese eel" 100 : 193-201, 2011

11 YVES COMBARNOUS, "Molecular Basis of the Specificity of Binding of Glycoprotein Hormones to Their Receptors" The Endocrine Society 13 (13): 670-691, 1992

12 최지현, "Molecular Analysis and Bioactivity of Luteinizing Hormone from Japanese Eel, Anguilla japonica, Produced in Silkworm Pupae" 한국생물공학회 21 (21): 381-388, 2016

13 H. Kagawa, "Mechanism of oocyte maturation and ovulation and its application to seed production in the Japanese eel" Springer Nature 39 (39): 13-17, 2013

14 Yukinori Kazeto, "Japanese Eel Follicle-Stimulating Hormone (Fsh) and Luteinizing Hormone (Lh): Production of Biologically Active Recombinant Fsh and Lh by Drosophila S2 Cells and Their Differential Actions on the Reproductive Biology1" Oxford University Press (OUP) 79 (79): 938-946, 2008

15 Maeda S, "Insect cell biotechology" CRC Press 1-31, 1994

16 Fortaine M, "Gonadal maturation of female eel Anguilla anguilla and spontaneous emission of eggs in aquarium" 259 : 2907-2910, 1964

17 Hiroyasu Kamei, "Expression of a biologically active recombinant follicle stimulating hormone of Japanese Eel Anguilla japonica using methylotropic yeast, Pichia pastoris" Elsevier BV 134 (134): 244-254, 2003

18 Nagahama Y., "Endocrine regulation of gametogenesis in fish" 38 : 217-229, 1994

19 Hirohiko Kagawa, "Effects of Rearing Period in Seawater on Induced Maturation in Female Japanese Eel Anguilla japonica" Japanese Society of Fisheries Science 64 (64): 77-82, 1998

20 Klein J, "Development and charaterization of a long-acting recombinant hFSH agonist" 18 : 50-56, 2003

21 Kiichiro YAMAMOTO, "Cytological changes in the adenohypophysis of fresh-water cultivated male Japanese eels, Anguilla japonica, induced to maturation by transfer to sea water and Synahorin injection" Japanese Society of Fisheries Science 39 (39): 585-594, 1973

22 Vischer HF, "Both recombinant African catfish LH and FSH are able to activate the African catfish FSH receptor" Bioscientifica 31 (31): 133-140, 2003

23 Min KS, "Biological activities of tethered equine chorionic gonadotropin (eCG) and its deglycosylated mutants" 50 : 297-304, 2004

24 S. Legardinier, "Biological activities of recombinant equine luteinizing hormone/chorionic gonadotropin (eLH/CG) expressed in Sf9 and Mimic insect cell lines" Bioscientifica 34 (34): 47-60, 2005

25 P. Swanson, "Biochemistry and physiology of fish gonadotropins" Springer Nature 28 (28): 53-59, 2003

26 Shin DH, "Biochemical and histological studies on egg quality in the Japanese eel Anguilla japonica" Graduate School of Fisheries Science, Hokkaido University 2004

27 Hirohiko Kagawa, "In Vitro Effects of 17α-hydroxyprogesterone and 17α, 20β-dihydroxy-4-pregnen-3-one on Final Maturation of Oocytes at Various Developmental Stages in Artificially Matured Japanese Eel Anguilla japonica" Japanese Society of Fisheries Science 61 (61): 1012-1015, 1995