Comparison Study on Changes of Antigenicities of Egg Ovalbumin Irradiated by Electron Beam or X-Ray

다국어 초록 (Multilingual Abstract)

This study was conducted to compare the effects of two forms of radiation (electron and X-ray; generated by an electronbeam accelerator) on the conformation and antigenic properties of hen’s egg albumin, ovalbumin (OVA), which was usedas a model protein. OVA solutions (2.0 mg/mL) were individually irradiated by electron beam or X-ray at the absorbeddoses of 0 (control), 2, 4, 6, 8, and 10 kGy. No differences between the two forms of radiation on the structural propertiesof OVA were shown by spectrometric and electrophoretic analyses. The turbidity of OVA solution increased and the mainOVA bands on polyacrylamide gels disappeared after irradiation, regardless of the radiation source. In competitive indirectenzyme-linked immunosorbent assay, OVA samples irradiated by electron beam or X-ray showed different immunologicalresponses in reactions with monoclonal and polyclonal antibodies (immunoglobulin G) produced against non-irradiatedOVA. The results indicate that electron beam irradiation and X-ray irradiation produced different patterns of structuralchanges to the OVA molecule.

참고문헌 (Reference)

1 이주운, "방사선 식품조사 기술과 식품 알레르겐의 구조 변화" 대한천식알레르기학회 23 (23): 16-23, 2003

2 Thayer, D. W, "Wholesomeness of irradiated foods" 48 : 58-67, 1994

3 Chan, J. K, "Thermal aggregation of myosin subfragments from cod and herring" 58 : 1057-1061, 1993

4 Lee, J. W, "The changes of allergenic and antigenic properties of egg white albumin (Gal d 2) by gamma irradiation" 30 : 500-504, 2001

5 SAS, "SAS/STAT Software for PC. Release 9.2"

6 Cleland, M. R, "Radiation processing with high-energy X-rays" 84 : 91-99, 2013

7 Hill, H. D, "Protein determination using bicinchoninic acid in the presence of sulfhydryl reagents" 170 : 203-208, 1988

8 Seo, J. H, "Ovalbumin modified by gamma irradiation alters its immunological functions and allergic responses" 7 : 464-472, 2007

9 Filali-Mouthim, A, "Lysozyme fragmentation induced by gamma-radiolysis" 72 : 63-71, 1997

10 Byun, M. W, "Information channel effects on women intention to purchase irradiated food in Korea" 78 : 675-677, 2009

11 Kume, T, "Immunochemical identification of irradiated chicken eggs" 65 : 1-4, 1994

12 Miller, R. B, "Food irradiation using bremsstrahlung X-rays" 68 : 963-974, 2003

13 Lee, J. W, "Effects of gamma radiation on the allergenic and antigenic properties of milk proteins" 64 : 272-276, 2000

14 Byun, M. W, "Effects of gamma irradiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp" 63 : 940-944, 2000

15 Kuntz, E, "Effects of electron beam irradiation on collagen" 20 : 376-391, 1961

16 Palou, L, "Effects of X-ray irradiation and sodium carbonate treatments on postharvert Penicillium decay and quality attributes of clementine mandarins" 46 : 252-261, 2007

17 Lee, J. W, "Conformational changes of myosin by gamma irradiation" 58 : 271-277, 2000

18 Van Calenberg, S, "Comparison of the effect of X-ray and electron beam irradiation on the microbiological quality of foodstuffs" 32 : 372-376, 1999

19 Van Calenberg, S, "Comparison of the effect of X-ray and electron beam irradiation on some selected spices" 31 : 252-258, 1998

20 Laemmli, U. K, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4" 227 : 680-685, 1970

21 Cho, K. H, "Changes of binding ability of milk-hypersensitive patients’ IgE to gamma-irradiated milk proteins" 30 : 505-509, 2001

22 Myung-Woo Byun, "Changes of Allergenicity and Conformational Structure of Egg Ovomucoidby Gamma Irradiation in the Basic Condition" 한국식품영양과학회 7 (7): 52-56, 2002

23 Kim, M. J, "Changes in the antigenic and immunoglobulin E-binding properties of hen’s egg albumin with the combination of heat and gamma irradiation treatment" 65 : 1192-1195, 2002

24 Kume, T, "Changes in structural and antigenic properties of proteins by radiation" 46 : 225-231, 1995

25 Al-kahtani, H. A, "Amino acid and protein changes in tilapia and Spanish mackerel after irradiation and storage" 51 : 107-114, 1998