아미노산 에스테르의 벤조피논 이민 유도체의 액체 크로마토그래피의 광학분리

다국어 초록 (Multilingual Abstract)

A convenient liquid chromatographic enantiomer separation of several α-amino acid esters as benzophenone Schiff base derivatives on covalently immobilized chiral stationary phases (CSPs) derived from polysaccharide derivatives was developed. The benzophenone imine derivatives of α-amino acid esters were readily prepared by stirring benzophenone imine and the α-amino acid ester hydrochloride salts in 2-propanol. The chromatographic conditions used on all CSPs were 0.5% or 5% 2-propanol/hexane (V/V) as the mobile phases at 1 mL/min of flow rate and UV 254 nm detection. The performance of Chiralpak IC among all CSPs was superior to that of the other CSPs for resolution of benzophenone imine derivatives of α-amino acid esters. It is expected that the developed analytical method will be useful for enantiomer resolution of other α-amino acid esters as benzophenone Schiff base derivatives.

목차 (Table of Contents)

• Abstract
• 1. 서론
• 2. 재료 및 실험방법
• 3. 결과
• 4. 결론
• References

참고문헌 (Reference)

1 황호, "다당 유도체를 기초로 한 키랄 컬럼에서 아미노산 에스테르의 안트르알디민 유도체의 광학분리 및 광학순도 측정" 한국생물공학회 26 (26): 139-142, 2011

2 서문준, "고성능 액체 크로마토그래피에 의한 다당 유도체를 기초로 한 흡착되거나 공유결합된 키랄 고정상에서 키랄 아미노 알코올의 안트르알디민 유도체 의 광학분리" 한국생물공학회 26 (26): 323-327, 2011

3 Greene, T. W, "Protective Groups in Organic Synthesis, 3rd ed" John Wiley & Sons 1999

4 Duchateau, A. L. L, "High-performance liquid chromatography of diamine enantiomers as Schiff bases on a chiral stationary phase" 664 : 169-176, 1994

5 Jing Yu Jin, "Enantiomer Separation of N-Protected α-Amino Acids on Covalently Immobilized Cellulose Tris(3,5-chlorophenylcarbamate) Chiral Stationary Phase in HPLC" 대한화학회 29 (29): 491-493, 2008

6 김경옥, "Covalently bonded and coated chiral stationary phases derived from polysaccharide derivatives for enantiomer separation of N-fluorenylmethoxycarbonyl alpha-amino acids with fluorescence detection" TAYLOR FRANCIS INC 29 (29): 1793-1801, 200605

7 이원재, "Comparative Studies Between Covalently Immobilized and Coated Chiral Stationary Phases Based on Polysaccharide Derivatives for Enantiomer Separation of N-Protected alpha-Amino Acids and Their Ester De" WILEY-LISS 21 : 871-877, 200910

8 Francotte, E, "Chirality in Drug Research" Wiley-VCH 2006

9 Subramanian, G., "Chiral Separation Techniques: A practical approach second revised ed" VCH 2001

10 O’Donnell, M. J, "A mild and efficient route to schiff base derivatives of amino acids" 47 : 2663-2666, 1982