벌크의 산소농도가 폐기물의 질산화 및 탈질에 미치는 영향 : Tracer 로서의 15N 동위원소 및 FISH 법을 이용한 아산화질소발생원의 규명 Study on sources of released nitrous oxide using 15N- isotope as a tracer and FISH method = Effects of oxygen in the bulk of refuses on nitrification and denitrification

다국어 초록 (Multilingual Abstract)

Nitrification and denitrification are important processes in the landfill site as they are deeply related with degradation and stabilization of refuse. Also nitrous oxide (N_2O) which is released from both nitrification and denitrification is known as greenhouse gas (GHG). The purpose of this study was to clarify the process by which N_2O produced using ^15N isotope. Nitrate which was labeled to 10.08% with ^15KNO_3 was used and N_2O was analyzed with GC mass. Results was that even also when O_2 of bulk was 15%, N_2O was released from denitrification. And as concentration of O_2 increase, sum of N_2O was released from denitrification. And as concentration of O_2 increase, sum of N_2O and N_2 was decreased and ratios of N_2O in the reduced gases were increased.
FISH technics also adaped to confirm whether which of nitrifiers existed in the substrates. When NEU was used of which the target was ammonia oxidizing bacteria, nitrifier was not detected at all. So it was confirmed that during the reaction denitrification was dominant process. Total bacteria distributions which were detected by EUB probe explained that as O_2 increase the number of bacteria also increase, but between the 10-15% of O_2 there was no any differences.