후각초성세포가 골수간엽줄기세포의 분화에 미치는 영향 = Effect of Olfactory Ensheathing Cells on Differentiation of Bone Marrow Stem Cells

다국어 초록 (Multilingual Abstract)

Olfactory ensheathing cells(OECs) transplantation is a promising orpotential therapy for spinal cord injury(SCI). Bone marrow stromal cells(BMSCs) contain mesenchymal stem cells and progenitor cells. BMSCs can differentiate into various cell types, including muscle and brain cells. In this study, we examined the feasibility of using BMSCs as a source of stem cells for the differentiation into OECs by co-culture approach. Only OECs and BMSCs were cultured as control group. The experimental group designed that OECs were transferred on transwell and insert this OECs culturing were co-cultured with BMSCs attached on 6 well bottom. Another experimental group cultured BMSCs in OEC-derived conditioned medium. Cultured cells were fixed at co-culture day 3, 7, 14 and 21. The expression of GFAP and P75 were characterized by immunocytochemistry. RT-PCR was used to evaluate the gene expression of P75, NSE and NF in BMSCs co-cultured with OECs. Our results showed that BMSCs able to differentiate into OEC-like cells using co-culture system in vitro. These findings could be helpful for the development of the cell-based therapeutic strategies for CNS repair.

참고문헌 (Reference)

1 김초민, "슈반세포가 골수간엽줄기세포의 분화에 미치는 영향" 한국조직공학과 재생의학회 4 (4): 60-66, 2007

2 최진산, "골수유래 간엽줄기세포와 점착성 단백질 및 폴리펩타이드가 흡착된(락티이드/글리콜라이드) 공중합체 지지체와의 상호작용" 한국고분자학회 27 (27): 397-404, 2003

3 김선경, "골수간엽줄기세포 및 다공성 지지체를 이용한 조직공학적 척추신경재생" 한국조직공학과 재생의학회 2 (2): 130-137, 2005

4 JG Boyd, "olfactory ensheathing cells do not associate with myelinated axons after implantation into the compressed spinal cord" 101 : 2162-, 2004

5 A Mahmood, "Treatment of traumatic brain injury in adult rats with intravenous administration of human bone marrow stromal cells" 53 : 697-, 2003

6 AI Caplan, "The mesengenic process" 3 : 426-, 1994

7 S Lou, "The effect of bone marrow stromal cells on neuronal differentiation of mesencephalic neural stem cells in Sprague-Dawley rats" 968 : 114-, 2003

8 R McKay, "Stem cells in the central nervous system" 276 : 671-, 1997

9 Y Li, "Repair of adult rat corticospinal tract by transplants of olfactory ensheathing cells" 277 : 2000-, 1997

10 MK Majumdar, "Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells(MSCs) and stromal cells" 176 : 57-, 1998

11 L Cao, "Olfactory ensheathing cells promote migration of Schwann cells by secreted nerve growth factor" 55 : 897-, 2007

12 HH Nash, "New method of purification for establishing primary cultures of ensheathing cells from the adult olfactory bulb" 34 : 81-, 2001

13 S Nef, "Neurotrophins are not required for normal embryonic development of olfactory neurons" 234 : 80-, 2001

14 EJ Kim, "Muscle-derived stem cell differentiate into neuronal cells for nerve regeneration" 1 : 41-, 2004

15 GC Kopen, "Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains" 14 : 10711-, 1999

16 CP Hofstetter, "Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery" 99 : 2199-, 2002

17 CA Ramon, "Long distance axonal regeneration in the transected adult rat spinal cord is promoted by olfactory ensheathing glia transplants" 18 : 3803-, 1998

18 MM Yaghoobi, "Differential gene expression pattern of neurotrophins and their receptors during neuronal differentiation of rat bone marrow stromal cells" 397 : 149-, 2006

19 SE Haynesworth, "Cytokine expression by human marrow-derived mesenchymal progenitor cells in vitro: effects of dexamethasone and IL-1 alpha" 166 : 585-, 1996

20 RF Pereira, "Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage, and lung in irradiated mice" 92 : 4857-, 1995

21 S Kadiyala, "Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro" 6 : 125-, 1997

22 H Castro-Malaspina, "Characterization of human bone marrow fibroblast colony-forming cells(CFU-F) and their progeny" 56 : 289-, 1980

23 HA Awad, "Autologous mesenchymal stem cell-mediated repair of tendon" 5 : 267-, 1999

24 JE Dennis, "A quadripotential mesenchymal progenitor cell isolated from the marrow of an adult mouse" 14 : 700-, 1999