Genome-wide identification and expression pattern of short-wavelength light responsive members of the NAC family in turnip

다국어 초록 (Multilingual Abstract)

The NAC transcription factor family plays a crucial role in stress response, plant development, and anthocyanin pigmentation. In turnip, anthocyanin biosynthesis is induced by UV-A or blue + UV-B light. However, characterization of turnip NACs is lacking, and whether they are involved in light-dependent anthocyanin accumulation is still unknown. In this study, we identified 200 BrNAC transcription factors in the turnip genome. They were intensively distributed on chromosomes A03, A07, A09, and A10. Phylogenetic analysis classified the turnip BrNAC gene family into 11 subfamilies. The protein subdomains were highly conserved between turnip and Arabidopsis thaliana, and exhibited a close evolutionary relationship. Protein-protein interaction prediction and RNA-seq expression profiles illustrated that the UV-A light responding genes BrNAC183, BrNAC195, and the blue + UV-B light responding gene BrNAC184 were the candidate genes associated with light-dependent anthocyanin accumulation in turnip. We discussed the findings on the relationship between the structure and function of the short-wavelength light responsive BrNAC family proteins from our results and the published data. Our results will aid further functional analysis of BrNAC family transcription factors. This research supports that BrNAC transcription factors regulate light-dependent anthocyanin biosynthesis in turnip.

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