9,10-dimethy1-1,2-benzanthracene (DMBA)으로 유발한 흰쥐의 악성 섬유성 조직구종의 세포주기에 관한 검색 = Cell Cycle Analysis of 9,10-dimethy1-1,2-benzanthracene (DMBA) Induced MFH Cell Strain of Wistar Rat

다국어 초록 (Multilingual Abstract)

Background/Objective Although a malignant fibrous histiocytoma (MFH) is the most common malignant soft tissue tumor in adults, its biologic property is not fully understood. The aim of the study is to present the basic data for cell cycle of MFH which is essential to elucidate the biologic property of component cells in experimentally-induced MFH. Method Experimental MFH was induced by repeated intra-articular injection of 9,10-dimethyl-1,2-benzanthracene (DMBA) in Wistar rats. Using the produced MFH, cell culture and cloning were performed, followed by establishment of a cell strain, in which radiolabelled thymidine was incorporated into DNA of proliferating cells by single exposure of tritiated thymidine. Results Average labelling index of DMBA-induced MFH cells was 26.6% and mitotic index was 4.3%. Labelled mitosis index (LMI) showed a maximum level(90.9%) at 4 hours incorporation. After decrease of LMI at 14 hours (7.8%), it's level again increased at 24 hours (56.8%). Each phase of cell cycle time was determined in the curve using Quastler and Sherman method. Conclusion A total generation time (TG) of DMBA induced MFH cell strain was about 24.1 hours, and the duration of S, G2, M, and G1 phase was 6.4, 2.0, 1.6, and 14.1 hours, respectively.