Competitive PCR 법을 이용한 Marine birnavirus ( MABV ) 의 정량적 검출 = Quantitative detection of Marine birnavirus ( MABV ) by competitive PCR

다국어 초록 (Multilingual Abstract)

It has been shown that TCID_50 and plaque assay might be hard to apply for the analysis of small amount of virus. Additionally polymerase chain reaction(PCR) does reply the quantity of virus correctly. Thus we developed the competitve PCR, and effective mean to investigate fish pathogenic virus, and analyzed the sensitivity against marine birnavirus (MABV). In this study, the virus sample of 1.44×10^7PFU/㎖ was detected after up to 10^4 dilution. And the existence of 100 copies MABV cDNA was identified with the competitive PCR in this virus sample. These results indicated that the indirect presumption was available on the basis of the amounts the viral genome. Viral genome`s number in the infected individual can be used as the fundamental data to divide the infection stage and to monitor the progress of disease.