Random Amplification of Polymorphic DNA(RAPD)를 이용한 Yersinia enterocolitica의 PCR Fingerprinting비교 = Comparative Study of PCR Fingerprinting of Yersinia enterocolitica Used by Random Amplification of Polymorphic DNA(RAPD)

다국어 초록 (Multilingual Abstract)

The lamer single primer was used to generate random amplification of polymorphic DNA(RAPD) profiles by polymerase chain reaction(PCR) for 100 strains of Y. enterocolitica from various sources. The RAPD pattern within same serotype was devided into different subtypes and also RAPD pattern of different serotypes showed same RAPD type. Regardless of serotype for Y. enterocolitica, DNA fingerprinting bands of non-pathogenic strains were a few in number and small in size but those of pathogenic were reversed. Every serotype showed unique RAPD pattern. therefore it is possible to discriminate within Y. enterocolitica strains. Showing some strains no band or a few, RAPD is effective method to subtype the strains. If RAPD was utilized with serotyping and biotyping, it should be powerful tool to devide Y. enterocolitica strains for epidem iological purpose.