글루텐분해유산균 GLU70 섭취에 따른 글리아딘 투여 마우스의 장내 염증성 사이토카인 및 유전자 발현의 변화 = Changes in intestinal inflammatory cytokine and gene expression in the intestines of gliadin-administered mice following gluten-degrading Lactobacillus strain GLU70 intake

다국어 초록 (Multilingual Abstract)

Excessive intake of wheat flour can lead to digestive disorders and intestinal diseases due to gliadin in gluten, an indigestible protein that is a causative agent of inflammation. This study aimed to confirm the effects of glutendegrading Lactobacillus strain GLU70 intake on the intestines of gliadin-administered mice. The results showed that GLU70 intake increased the activity of pancreatic proteases and significantly alleviated gliadin-induced damage in the jejunum tissue. While reducing the expression of TMF-α in the intestine, GLU70 intake increased the expression of the anti-inflammatory cytokine IL-10. It also reduced the mRNA expression of cytokines IL-4 and IL-5, which are related to allergic diseases, and decreased the expression of TGM2, zonulin, and p65NF-κB, which are proteins related to celiac disease, leaky gut syndrome, and inflammatory response respectively. Therefore, gluten-degrading Lactobacillus strain GLU70 may be useful in the food industry for its effects of alleviating gliadin-induced complications.

참고문헌 (Reference)

1 Sabença C, "Wheat/gluten-related disorders and gluten-free diet misconceptions : a review" 10 : 1765-1788, 2021

2 Levendoski EE, "Vocal fold epithelial barrier in health and injury : a research review" 57 : 1679-1691, 2014

3 Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)., "Trend Report in the field of postbiotics. 2022 Food R&D Key Investment Area Trend Report" 2022

4 Rubio-Tapia A, "The prevalence of celiac disease in the United States" 107 : 1538-1544, 2012

5 Yoon JY, "Studies on identification, purification and characteristics of gluten degradable enzyme isolated from Lactobacillus paracasei" 22 : 18-24, 2017

6 Luyer MD, "Strain-specific effects of probiotics on gut barrier integrity following hemorrhagic shock" 73 : 3686-3692, 2005

7 Ferrari E, "Probiotics supplements reduce ER stress and gut inflammation associated with gliadin Intake in a mouse model of gluten sensitivity" 13 : 1221-1233, 2021

8 Abraham BP, "Probiotics in inflammatory bowel disease" 46 : 769-782, 2017

9 Huang X, "Oxidative modification of a proline-rich gliadin peptide" 141 : 2011-2016, 2013

10 Leon B, "Modulating Th2 cell immunity for the treatment of asthma" 12 : 637948-637961, 2021

11 Cardoso-Silva D, "Intestinal barrier function in glutenrelated disorders" 11 : 2325-2343, 2019

12 Glocker EO, "IL-10 and IL-10 receptor defects in humans" 1246 : 102-107, 2011

13 Cabanillas B, "Gluten-related disorders : Celiac disease, wheat allergy, and nonceliac gluten sensitivity" 12 : 1-16, 2019

14 Kõiv V, "Gluten-degrading bacteria : availability and applications" 105 : 3045-3059, 2021

15 Singh P, "Global prevalence of celiac disease : systematic review and meta-analysis" 16 : 823-836, 2018

16 Barone MV, "Gliadin peptides as triggers of the proliferative and stress/innate immune response of the celiac small intestinal mucosa" 15 : 20518-20537, 2017

17 Freitag TL, "Gliadin nanoparticles induce immune tolerance to gliadin in mouse models of celiac disease" 158 : 1667-1681, 2020

18 Štepánková R, "Experimentally induced gluten enteropathy and protective effect of epidermal growth factor in artificially fed neonatal rats" 36 : 96-104, 2003

19 Bakshi A, "Emerging therapeutic options for celiac disease : Potential alternatives to a gluten-free diet" 8 : 582-588, 2012

20 Hollon J, "Effect of gliadin on permeability of intestinal biopsy explants from celiac disease patients and patients with non-celiac gluten sensitivity" 7 : 1565-1576, 2015

21 Quagliariello A, "Effect of Bifidobacterium breve on the intestinal microbiota of coeliac children on a gluten free diet : A Pilot Study" 8 : 660-675, 2016

22 de Almeida NEC, "Digestion of intact gluten proteins by Bifidobacterium species : Reduction of cytotoxicity and proinflammatory responses" 68 : 4485-4492, 2020

23 Fajgenbaum DC, "Cytokine storm" 383 : 2255-2273, 2020

24 정현나 ; 조효선, "Current status of microbiome-based therapeutics research and development" 59 : 159-172, 2023

25 Sánchez D, "Contribution of infectious agents to the development of celiac disease" 9 : 547-567, 2021

26 Ceballos C, "Complementary and alternative medicine use at a single pediatric inflammatory bowel disease center" 37 : 265-271, 2014

27 Attarwala HZ, "Co-silencing of tissue transglutaminase-2 and interleukin-15 genes in a Celiac disease mimetic mouse model using a naanoparticle-in-microsphere oral system" 18 : 3099-3107, 2021

28 Wieser H, "Chemistry of gluten proteins" 24 : 115-119, 2007

29 황연지 ; 채인숙 ; 이윤경, "Anti-inflammatory effects of fermented Laminaria japonica and Hizikia fusiforme water extracts with probiotics in LPS-stimulated RAW264.7 macrophage cell line" 27 : 1-8, 2017

30 Jenmalm M, "Aller-gen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children" 31 : 1528-1535, 2001

31 Klemenak M, "Administration of Bifidobacterium breve decreases the production of TNFalpha and faecal fermentation index in children with celiac disease" 60 : 3386-3392, 2015

32 Dowlati Y, "A meta-analysis of cytokines in major depression" 67 : 446-457, 2010