형태형질에 기초한 동아시아산 황기속 식물의 계통분류 = Phylogenetic study of East-Asia Astragalus L. based on morphological characters

다국어 초록 (Multilingual Abstract)

In order to find useful taxonomic morphological characters, 17 taxa from East-Asia Astragalus and Oxytropis anertii were investigated. In this study, seed coat structure, fruit surface, and fruit anatomical structures were selected to classify the species in Astragalus. As a results, useful morphological and anatomical characters in East-Asia Astragalus were life cycle, leaflet shape, raceme, petal color, seed color, pod shape, absent or present of hairs in pod surface, bent degree of dorsal suture, and thickness of endocarp. Using medicinal sources in China, A. floridus, A. membranaceus, A. membranaceus var. mongholicus and A. tongolensis were formed same group. However, these were not completely consensus in the morphological characters. The other hand, A. koraiensis and A. sikokianus, which have controversial taxonomic issue, have clearly no differences morphological characters each other. Therefore, A. koraiensis, A. sikokianus, and A. bhotanensis should be conducted a comparative study on the DNA sequences and molecular cytology in order to clarify the taxonomic treatments in the near future.

국문 초록 (Abstract)

동아시아산 황기속의 외부형태학적 특징과 과피, 종피의 해부구조 및 표면 형태를 조사하여 분류학적으로 유용한 형질을 찾고자 하였다. 연구 결과, 황기속의 외부형태, 과피, 종피의 형태 및 해부학적 특징 중 생활사, 소엽의 형태, 꽃차례, 꽃잎의 색, 종자의 색, 과피의 형태, 과피 표면에서 털의 존재유무, 봉선의 구조, 그리고 내과피 층의 두께가 종을 나누는 식별형질로 사용될 수 있음을 확인하였다. 이를 바탕으로 중국에서 황기라는 약재로 사용되어지고 있는 4종의 형태 및 해부학적인 특성을 비교한 결과, 서로 같은 군으로 유집되었다. 한편, 분류학적으로 논쟁이 되고 있는 정선황기와 갯황기에서는 두 분류군간에 형태적 차이점을 발견 할 수 없었다. 따라서 금후 정선황기, 갯황기, A. bhotanensis의 종간 분류학적 처리를 명확히 규명하기 위하여 염기서열 및 분자세포학 수준에서의 연구가 필요하다.

참고문헌 (Reference)

1 Liston, A, "The phylogenetic position of the genus Astragalus (Fabaceae): evidence from the chloroplast genes rpoC1 and rpoC2" 22 : 377-388, 1994

2 Yaklich, R. W, "The fine structure of aleurone cells in the soybean seed coat" 167 : 108-119, 1992

3 Park, J. W, "The Genera of Vascular Plant of Korea. Flora of Korea Editorial Committee"

4 Kim, S. Y, "Taxonomic study on the genus Astragalus (Leguminosae)in Korea" Inha Univ 2004

5 최인수, "Taxonomic position and genetic differentiation of Korean Astragalus mongholicus Bunge" 한국식물분류학회 43 (43): 12-21, 2013

6 Hong, L. W, "Structure and development of Medicago truncatula pod wall and seed coat" 95 : 737-747, 2005

7 Ma, X. Q, "Species identification of radix Astragali (Huangqi)by DNA sequence of its 5S-rRNA spacer domain" 54 : 363-368, 2000

8 Al-gohary, I. H, "Seed morphology of Acacia in Egypt and its taxonomic significance" 9 (9): 435-438, 2007

9 Riahi, M, "Seed development in Astragalus cemerinus and A. ruxcifolius (Fabaceae), and ITS systematic implications" 51 : 111-117, 2009

10 Moise, J. A, "Seed coats: structure, development, composition, and biotechnology" 41 : 620-644, 2005

11 박희운, "SCAR 마커를 이용한 황기와 몽고황기 판별" 한국약용작물학회 15 (15): 51-55, 2007

12 방경환, "RAPD마커를 이용한 황기의 유전적 다양성 및 기원판별" 한의병리학회 18 (18): 825-829, 2004

13 Lee, C. B, "Plant Taxonomy" Hyangmunsa Press 1985

14 Dong, T. T. X, "Phylogeny of Astragalus in China: molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA" 51 : 6709-6741, 2003

15 Lee, Y. N, "New taxa of Korean flora" 24 : 27-30, 1981

16 Osaloo, S. K, "Molecular systematics of the genus Astragalus L. (Fabaceae): Phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndhF sequence" 242 : 1-32, 2003

17 Mabberley, D. J, "Mabberley's plant book. 3rd edition" 77 : 474-, 2008

18 Lock, J. M, "Legumes of West Asia, a check list" Royal Botanical Gerdens 1991

19 Chung, T. H, "Korean Flora" Sinjisa 336-339, 1957

20 Lee, C. B, "Illustrated Flora of Korea" Hyangmunsa Press 491-492, 1980

21 Toh, C. A, "Histological comparison of Astragalus membranaceus vs. Astragalus membranaceus var. alpinus" Ehwa Womans University 1959

22 Im, R. J, "Flora of Coreana" Science and Technology Publishing House Pyongyang 115-120, 1975

23 Sanderson, M. J, "Diversification rates in a template legume clade: are there so many species of Astragalus (Fabaceae)" 83 : 1488-1502, 1996

24 Lee, W. T, "Coloured Standard Illustrations of Korean Plants" Academy Press 555-558, 1996

25 Tiwari, S. P, "Characters of pod anatomy associated with resistance to pod-shattering in soybean" 76 : 483-485, 1995

26 Toh, C. A, "A cytotaxonomic study on the Astragalus membranaceus and Astragalus membranaceus var. alpinus" 3 : 57-61, 1971