근수축시 해당작용에 의한 근형질 세망의 Ca²⁺ 변화가 미토콘드리아 Ca²⁺ 증가에 미치는 영향 = Glycolysis Mediated Sarcoplasmic Reticulum Ca²⁺ Signal Regulates Mitochondria Ca²⁺ during Skeletal Muscle Contraction

다국어 초록 (Multilingual Abstract)

PURPOSE: This study was to investigate the Glycolysis mediated sarcoplasmic reticulum (SR) Ca²⁺ signal regulates mitochondria Ca²⁺ during skeletal muscle contraction by using glycolysis inhibitor.
METHODS: To examine the effect of Glycolysis inhibitor on SR and mitochondria Ca²⁺ content, we used skeletal muscle fiber from gastrocnemius muscle. 2-deoxy glucose and 3-bromo pyruvate used as glycolysis inhibitor, it applied to electrically stimulated muscle contraction experiment. Intracellular Ca²⁺ content, SR, mitochondria Ca²⁺ level and mitochondria membrane potential (MMP) was detected by confocal microscope. Mitochondrial energy metabolism related enzyme, citric acid synthase activity also examined for mitochondrial function during the muscle contraction.
RESULTS: Treatment of 2-DG and 3BP decreased the muscle contraction induced SR Ca²⁺ increase however the mitochondria Ca²⁺ level was increased by treatment of inhibitors and showed and overloading as compared with the control group. Glycolysis inhibitor and thapsigargin treatment showed a significant decrease in MPP of skeletal muscle cells compared to the control group. CS activity significantly decreased after pretreatment of glycolysis inhibitor during skeletal muscle contraction. These results suggest that regulation of mitochondrial Ca²⁺ levels by glycolysis is an important factor in mitochondrial energy production during skeletal muscle contraction
CONCLUSIONS: These results suggest that mitochondria Ca²⁺ level can be regulated by SR Ca²⁺ level and glycolytic regulation of intraocular Ca²⁺ signal play pivotal role in regulation of mitochondria energy metabolism during the muscle contraction.