비교 유전자 보합법을 이용한 한국인 갑상선 유두상 암종에서의 유전자 변화에 관한 연구 = Analyses of Genetic Alterations in Papillary Thyroid Carcinomas by Comparative Genomic Hybridization in Korea

다국어 초록 (Multilingual Abstract)

Transformation and progression of cancers are thought to be caused by an accumulation of complex genetic alterations, but little is known about specific genetic changes. In this study, the authors have undertaken a genome-wide screening to detect changes in 12 cases of papillary thyroid carcinoma which is the most common subtype of thyroid cancer among Koreans. Comparative genomic hybridization (CGH) was used to screen for DNA sequence gains and losses across all human chromosomes. Biotin-labeled tumor DNA and digoxigenin-labeled normal DNA were hybridized to normal metaphase cells. The fluorescence signals were captured by fluorescence microscope after detection by avidin FITC and antidigoxigenin rhodamine. Then, the ratio of fluorescence was calculated by an image analyzer.
The CGH results showed gains on chromosomes 1q(25%), 13q(25%), 17q(25%), 7p(17%), 18q(17%), 17p(8%) and 7q(8%). The Deletions were on chromosomes 22q(25%), 19p(8%), 19q(8%), 20p(8%), and 20q(8%). Usual amplification sites of genome were 1q, 13q, and 17q. The most common deletion site was 22q. The results indicate that the amplification on chromosome 1q, 13q, and 17q and the deletion on chromosomes 22q are the most frequent genetic alterations in papillary thyroid carcinoma among Koreans. The CGH was thought to be very useful in the screening of genetic alterations of solid tumors.