Cisplatin이 흰쥐 십이지장의 alkaline phosphatase 활성에 미치는 영향에 대한 연구 = Effect of Cisplatin on Alkaline Phosphatase Activity of Duodenum in Rats

다국어 초록 (Multilingual Abstract)

Cisplatin is a platinum compound, which has been widely used as an anticancer chemotherapeutic agent. During the treatment of cancer patients cisplatin acts on normally proliferating cells, which results in nephrotoxicity, nausea, vomiting, anorexia, bone marrow suppression and inner ear damage. Cisplatin inhibits the DNA synthesis, protein synthesis and cell division by incorporation to DNA strands.
The author has investigated the effect of cisplatin on duodenum of rats histochemically observing the changes in the activity of alkaline phosphatase. The rats treated with 6mg per kg of cisplatin were sacrificed at 6 hours, 12 hours, 24 hours and 3 days after the drug administration. The rats of control group were administered only water for injection. The duodenum specimens were fixed in 10%-neutral formalin at 4 。and sectioned 16 。thickness in frozen cryostat. The activity of alkaline phosphatase was evaluated histochemically by Gomori's method and incubation time of sliced specimen was 30 minutes.
The results were as follows.
1. In control rats, the activity of alkaline phosphatase were moderate positive in the epithelium of mucosa and weak or trace positive in lamina propria, submucosa and muscle layer.
2. In the epithelium of the mucosa the activity of alkaline phosphatase were weak positive in 6 hours of cisplatin treated rats, moderate or strong positive in 12 hours and 3 days of cisplatin treated rats and strong positive in 24 hours of cisplatin treated rats.
3. There are trace or weak reactivities in the lamina propria, submucosa and muscle layer of duodenum of all groups.
Consequently, it is suggested that cisplatin reduces the activity of alkaline phosphatase at 6 hours after administration and the activities of alkaline phosphatase by regeneration and differentiation of epithelium in the duodenum of cisplatin treated rats.