택사(Alismatis rhizoma) 추출물의 MCF-7 유방암세포 증식 억제 효과 = The Inhibitory Effect of Alismatis Rhizoma Extract on the Proliferation of MCF-7 Breast Cancer Cells

국문 초록 (Abstract)

본 연구 목적은 택사(Alismatis rhizoma)추출물의 유방암 세포 성장 억제 효과와 그 기전을 규명하는 것이다. 택사(Alismatis rhizoma)추출물에 대해 유방암에도 의 성장 억제 효과는 WST-1분석법으로 측정하고, 관련 단백질 및 유전자에 미치는 영향은 Real-time PCR을 이용하여 분석하였다. 그 결과 택사(Alismatis rhizoma)은 유방암 세포의 성장을 유의하게 억제했으며, 억제 효과는 용량 및 처리 시간과 양의 상관관계가 있는 것으로 나타났다. 또한 택사(Alismatis rhizoma)은 세포 주기 단백질 D1(Cyclin D1)의 발현 수준을 유의하게 감소시켰다. 또한 택사(Alismatis rhizoma)은 세포의 이동 및 침입 능력을 억제다. 종합하면 택사(Alismatis rhizoma)은 유방암 세포의 성장을 억제할 수 있는 잠재력을 가지고 있으며 세포 주기를 조절하고 세포 이동 및 침입을 억제하는 방식으로 작용할 수 있다.

다국어 초록 (Multilingual Abstract)

This study aimed to investigate the inhibitory effect of Alismatis rhizoma on MCF-7 breast cancer cells and its underlying mechanisms. MTT and WST-1 assays were conducted to evaluate the growth inhibitory effect of Alismatis rhizoma on MCF-7 cells，and Real-time PCR were used to analyze its impact on relevant proteins and genes. The results showed that Alismatis rhizoma significantly suppressed the growth of MCF-7 cells, and the inhibition effect was positively correlated with dosage and treatment time. Furthermore, Alismatis rhizoma markedly decreased the expression levels of Cyclin D1. Additionally, Alismatis rhizoma inhibited the migration and invasion ability of the cells. In conclusion, Alismatis rhizoma possesses the potential to inhibit the growth of MCF-7 cells, possibly through regulating the cell cycle and suppressing cell migration and invasion.

목차 (Table of Contents)

• I. 서론············································································································ 1
• 1. 연구 배경 및 필요성····················································································· 1
• 2. 암 치료에 있어 천연 약물의 적용·································································· 2
• 3. 연구 목적·································································································· 3
• 4. 택사(Alismatis rhizoma)의 개요····························································· 4
• Ⅱ. 재료 및 방법······························································································· 6
• 1. 유방암 세포 소개························································································· 6
• 2. 유방암 세포 배양························································································ 6
• 3. WST-1 분석법··························································································· 7
• 4. Real-time PCR법····················································································· 9
• Ⅲ. 결과········································································································· 11
• 1. 택사(Alismatis rhizoma) 추출물에 의한 MCF-7 세포증식 변화 관찰 ············ 11
• 2. 택사(Alismatis rhizoma) 추출물에 의한 MCF-7 의 종양억제 단백질 및 종양 관련 사이토카인 유전자 발현 변화 관찰 ···· 14
• Ⅳ. 토론········································································································· 20
• Ⅴ. 결론·········································································································· 22
• 참고 문헌········································································································ 23
• 초록··············································································································· 27
• Abstract····································································································· 28