사육사슴 및 야생고라니의 혈청학적 조사를 통한 질병실태에 관한 연구 = Antibody Prevalence of Ruminant Infectious Diseases in Farmed Deer and Wild Water Deer

국문 초록 (Abstract)

제1편
Since the emergence of deer farming as an alternative farming industry, there has been an increasing awareness of infectious diseases which commonly occur in cattle. Deer can be one of the susceptible animals for bovine infectious diseases and can play a role as a reservoir, which can cause economic loss in cattle livestock industry. Serum samples were randomly collected from 78 deer from 31 farms at Chonbuk province, and 7 wild water deer from Chonbuk wild animal treatment center during 2005 to 2007, respectively. Hematology was analyzed. The prevalence of antibody were tested for 3 bacterial, 4 viral, 5 arthropod-borne diseases and neosporosis, which are infectious diseases in ruminants. Serum antibodies were present against tuberculosis (5.1%) and paratuberculosis (1.3%) in farmed deers, but no serum was detected for brucellosis. Among bovine viral diseases, only bovine leukosis was detected in farmed deers. Two farmed sika (2.6%) had antibodies to enzootic bovine leukosis. Serologic results of FMD, IBR, and BVD agents were negative. The positive rates for Akabane, Aino and Epizootic hemorrhagic disease (EHD) in the farmed deer were 80%, 62.2% and 55.6%, respectively, and those for Chuzan and bovine ephemeral fever (BEF) in the same deer groups were 15.6% and 4.4%, respectively. However, the wild water deer similarly showd that higher detection for Akabane and EHD and low for BEF and Chuzan. Antibodies to Neospora caninum were detected in sera of farmed deer(17.9%), but no wild deer was positive. The seropositive reaction was detected all of the investigated area, ranged from 6.7 to 30.7%.
On the basis of these results, deer is susceptible host for ruminant infectious diseases in housed indoor under intensive management system or a natural environment and these diseases may present a risk to other species, especially to domestic cattle. This is the first report for antibody seroprevalence of ruminant infectious disease for deer in Korea.
제2편
Serum samples collected from 78 deer from 31 farms at Chonbuk province and 7 wild water deer from Chonbuk wild animal treatment center during 2005 to 2007, respectively, were assayed for antibodies against enzootic bovine leukosis, foot and mouth disease (FMD), infectious bovine rhinotracheitis(IBR), and bovine viral diarrhea(BVD). Two serum antibodies were present against enzootic bovine leukosis(2.6%) for farmed sika deer. No serologic evidence of FMD, IBR, and BVD was found. On the basis of these limited results, farmed deer showed relatively low positive to viral disease which threaten to other ruminant species. But periodic observation and caution should be applied to farmed deer for prevention, detection, or infectious risk to other species.
제3편
Mosquito-borne diseases are primarily associated with reproductive disorders or acute febrile diseases in ruminants. Although mosquito-borne diseases have been generally reported in ruminants, no epidemiological studies in deer have been reported in Korea. Serum samples were randomly collected from 45 deer from 24 farms in Chonbuk province and 7 wild water deer from Chonbuk wild animal treatment center during 2005 to 2007, respectively, and were analyzed by virus neutralization assay (VNA). High antibody seroprevalence was 80.0% for Akabane, 62.2% for Aino, 55.6% for epizootic hemorrhagic disease (EHD) in the farmed deer, respectively. And 15.6% for Chuzan and 4.4% for bovine ephemeral fever (BEF) were low positive in serum at same deers. Similarly, the seroprevalences in wild water deer were positive in 6 deer to Akabane, 4 deer to EHD in the wild deer, and those to Chuzan and BEF were positive in 1, and 2 wild deer, respectively. No Anio positive was observed. The positive rates for Akabane, EHD and Chuzan were similar in both farm and wild deer, but those for Aino and BEF showed different based on VNA in the between groups. The results indicate that farmed deer and wild water deer are susceptible hosts of arthropod-borne diseases in a natural environment.
제4편
Serum samples were randomly collected from 78 deer from 31 farms and 7 wild water deer from wild animal medical center during 2005 to 2007, respectively. A total 85 sera were tested for Neospora. canium antibodies by ELISA. Fourteen farmed deer (17.9%) were positive to N. canium but no antibodies were found in sera from wild water deer. The sera from all of the area for these study showed positive in the range from 6.7 to 30.7%. On the basis of these study, farmed deer in Chonbuk province were exposed extensively and seriously to N. canium which might present a risk to other species included cattle and dog.

목차 (Table of Contents)

• Abstract = 1
• 서론 = 3
• 제1편 사슴에서의 세균성 전염병에 대한 혈청학적 연구 = 7
• Abstract = 7
• 서론 = 8
• 재료 및 방법 = 11
• 가. 공여혈청 = 11
• 나. 혈청학적 검사 = 11
• 결과 = 14
• 가. 일반혈액검사 = 14
• 나. 혈청학적 브루셀라 양성율 = 14
• 다. 혈청학적 우결핵 양성율 = 14
• 라. 혈청학적 요네병 항체 양성율 = 15
• 고찰 = 16
• 결론 = 19
• 제 2 편 사슴에서의 바이러스성 전염병에 대한 혈청학적 연구 = 26
• Abstract = 26
• 서론 = 27
• 재료 및 방법 = 30
• 가. 공여혈청 = 30
• 나. 혈청학적 검사 = 30
• 결과 = 33
• 가. 혈청학적 소류코시스 양성율 = 33
• 나. 혈청학적 구제역 양성율 = 33
• 다. 혈청학적 소전염성비기관지염 양성율 = 33
• 라. 혈청학적 소바이러스설사증 양성율 = 34
• 고찰 = 35
• 결론 = 39
• 제 3 편 사슴에서의 모기매개성 전염병에 대한 혈청학적 연구 = 42
• Abstract = 42
• 서론 = 44
• 재료 및 방법 = 46
• 가. 공여혈청 = 46
• 나. 혈청학적 검사 = 46
• 결과 = 48
• 가. 아까바네 항체가 = 48
• 나. 아이노병 항체가 = 48
• 다. 츄잔과 유행열 항체가 = 49
• 라. 유행성출혈열 항체가 = 49
• 고찰 = 50
• 결론 = 53
• 제 4 편 사슴에서의 Neospora caninum에 대한 혈청학적 연구 = 61
• Abstract = 61
• 서론 = 62
• 재료 및 방법 = 63
• 가. 공여혈청 = 63
• 나. 네오스포라에 대한 혈청학적 검사 = 63
• 결과 = 64
• 고찰 = 65
• 결론 = 66
• 결론 = 68
• 참고문헌 = 70