Hantaan virus에 감염된 등줄쥐 폐조직중의 Reovirus 검출 = Detection of Reovirus in the infected lung tissues of Apodemus agrarius with Hantaan virus

다국어 초록 (Multilingual Abstract)

The followings are the results of the detection of Reovirus in the infected lung tissues of Apodemus agrarius with Hantaan virus, the etiologic agent of Hemorrhagic fever with renal syndrome.
1. About one third of infected Apodemus agrarius jejuensis with Hantaan virus revealed Reovirus antigen in the lung tissues and Reovirus antigen showed the quite different immunofluorescent reaction pattern compare with Hantaan virus antigen.
2. It was almost impossible to detect least amount of reovirus antigen in the infected lung tissues of Apodemus agrarius with Hantaan virus by indirect IF antibody technique.
3. Reovirus growed rapidly than Hantaan virus and produced CPE when the Hantaan virus seed of Apodemus mice lungs containing undetectable amount of reovirus by indirect IF technique was passaged several times in A549 cell cultures.
4. The detection of reovirus antigen in the lungs of mice inoculated blood specimens from Hemorrhagic fever with renal syndrome patients was dependent upon individual mouse and the source of reovirus contamination in the Hantaan virus seed was not blood of patients.
These findings show that isolation of Hantaan virus from clinical materials and from infected Apodemus mice should be carefully done both in tissue culture cells and in normal Apodemus mice because about one third of Apodemus agrarius captured in nature were infected with Reovirus.