생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과 = Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity

다국어 초록 (Multilingual Abstract)

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

목차 (Table of Contents)

• 서론
• 재료 및 방법
• 실험재료
• 토끼 근위 세뇨관 상피세포의 분리 및 배양
• 인체 정상 신장세포의 분리 및 배양
• 세포독성 실험
• 삼차원적으로 조직 배양된 인체 신장 피질 조직에서의 glucose consumpation 시험
• 혈청 분리 및 BUN, Creatinine 측정
• DPPH assay
• Superoxide anion radical 형성에 미치는 영향
• XOD 활성저해효과
• 흰쥐 간 마이크로좀에서 지질 과산화 저해효과
• 통계처리
• 결과
• Cisplatin의 신장세포 독성에 대한 검체의 효능
• 인체 신장 조직 배양에서의 포도당 소모량에 대한 이공산의 영향
• Clisplatin 유도 흰쥐체중 및 혈청생화학적 지표에 미치는 이공산의 영향
• 이공산의 DHHP radical소거 작용
• Superoxide anion radical생성에 대한 영향
• XOD 활성 저해 작용
• 흰쥐 간 마이크로좀에서 지질과산화 저해작용
• 고찰