URP-PCR 분석에 의한 느타리 단핵 계통간 교잡주의 핵 DNA 유전

다국어 초록 (Multilingual Abstract)

The primary objective of the present study is the characterization of the hybrids of monokaryon- monokaryon (monomono) crosses in mushroom breeding. We employed this technique for developing superior strains from Pleurotus species strains with 85 mono-mono intraspecific hybrids of 7 combinations between six Pleurotus ostreatus strains and one Pleurotus florida strain. In this study, the results of analysis on hybridization rate, nuclear DNA patterns, and colors and yields of fruit-bodies, are presented as follows. The crossability between mono-mono crossing ranges between 50 and 93.75%. The results of the analysis on the nuclear DNA patterns of 85 hybrid strains of mono-mono crosses share the nuclei of both parents, but their genetic similarities were predominated by either parent. The hybrid strain between P. florida and P. ostreatus showed patterns more similar to P. florida, while the hybrid strain between P. ostreatus and P. ostreatus either had patterns predominated by either parent strain. The fruiting body colors of the mono-mono crosses mostly had combined colors of both parents but showed the tendency of being more similar to that of either parent. 82% of the hybrid strain indicated similar fruiting body yields compared to both parent strains, while 0% was higher and 18% were lower than both parents. The present study was able to find out and suggest superior hybrid trains by identifying the nuclear DNA patterns of hybrids between Pleurotus species as well as the characteristics of their fruiting bodies. This study expects that the advantages of the mono-mono crossing are needs to be fully utilized in mushroom breeding and it is better to develop superior strains of Pleurotus species strains together with the mono-mono crossing.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 균주 및 배양
• 단포자 분리 및 교배형 검정
• 단핵-단핵(mono-mono) 계통간 교잡
• DNA 다형성 분석
• 유전유사도 분석
• 교잡주의 자실체 특성분석
• 결과 및 고찰
• 적요
• 감사의 말씀
• 참고문헌

참고문헌 (Reference)

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