肝吸蟲感染 白鼠 에 있어서 Praziquantel(Embay 8440)의 殺蟲效果에 관한 實驗的 硏究 = Experimental study on the therapeutic effect of praziquantel (Embay 8440) in rats experimentally infected with Clonorchis sinensis

다국어 초록 (Multilingual Abstract)

Experimentally infected ats with about 50 metacercariae of Clonorchis Sinensis in each were treasted orally with different dosages of praziquantel. About 100 rats were divided into 8 groups in each developmental weeks after infection(from the first to 8th week after infection) and 24 rats served as untreated controls.
In each developmental weeks after infection, about 15 rats divided into 4 groups including control in each 3 to 5 rats were used to determine for the curative activity of praziquantel against C. sinensis. The experimentally infected rats in each developmental weeks after infection received praziquantel 1×100㎎/㎏×3 days, 3×100㎎/㎏×1 days and 3×100㎎/㎏×2 days.
The effects of the drug were evaluated by the worm recovery rates, morphological changes of the worms and number of eggs per gram of feces(EPG) after treatment.
The feces of individual rats were collected once in a week at one week before and 4 weeks after treatment, and the number of Clonorchis eggs were examined by Stoll's egg counting method. The necropsy was performed on 4 weeks after treatment. The liver and the bile ducts were examined immediately after the dissection, and all of the worms recovered from the liver immersed in warm saline solution in a small petri dish in order to observe the motility of indicidual worms. After that, all worms obtained from the treated and untreated control rats were fixed in 10% formalin solution. The whole mount specimens were prepared by Semichon's acetocarmine stain for the study of morphological changes.
In the treatment with dosage of 1×100㎎/㎏ for 3 consecutive days, relatively low worm recovery rates were shown in the early stages of infection than those of control groups. However there was no practically big differences of the worm recovery rates between the later stages of infection(7 to 8 weeks after infection) and control groups.
In the early stages of infection, the worms recovered in the bile ducts of each treated rats showed morphologically intact as a normal control groups, however in the later stages of infection the worms showed already damaged their reproductive organs such as testes, seminal receptacle and ovary etc.
In the treatment with dosage of 3×100㎎/㎏×1 days, the recovery rates of the worms in all developmental weeks after infection were considerable low and some growth inhibition of the worms were also noted. The worms recovered from the bile ducts in all developmental stages were seriously damaged in their reproductive organs, such as testes, seminal receptacle, ovary, Mehlis' gland, uterus and vitelline glands etc. The hyaline degenerations were also observed on the parenchymal tissues in the dead worms.
The mean EPG values in this group were markedly reduced at the first 2 weeks after treatment. However the low values of EPG were generally noted at the 3rd to 4th week after treatment.
On the other hand, in the treatment with dosage of 3×100㎎/㎏×2 days, no more the living worm was recovered in all developmental stages of Clonorchis. The dead worms recovered from the bile ducts were so seriously damaged that the morphological cahnges could not be observed. The mean EPG values in this group were markedly reduced during the first 2 weeks after treatment. At the 3rd week after treatment EPG value became negative in the most of all treated groups. No more the Clonorchis eggs were detected at the 4th week after infection, but only a very small numbers of eggs were found by the concentration method.
From the above results, it is obvious that the present study shows that praziquantel exhibits a high degree of activity against Clonorchis sinensis in the rats, with no apparent differences in the efficacy against each developmental stages.

목차 (Table of Contents)

• Ⅰ. 緖論
• Ⅱ. 實驗材料 및 方法
• Ⅲ. 試驗成績
• Ⅳ. 考察
• Ⅴ. 結論
• Ⅵ. 參考文獻