Molecular detection of Bartonella henselae DNA from fleas obtained from dogs, Korea

국문 초록 (Abstract)

목 적 : 소아 임파선염의 흔한 원인균의 하나로 잘 알려진 Bartonella henselae는 주로 고양이 벼룩에 감염된 고양이와의 접촉을 통하여 병을 일으키는 것으로 알려졌지만, 최근에는 개도 보유 숙주임이 확인되었다. 국내의 CSD증례 보고들에서는 고양이보다 개와 접촉한 경우가 더 많은 것으로 보고되었다. 하지만 국내에서는 개나 고양이 또는 매개체인 고양이 벼룩 등에서 B. henselae가 확인된 경우가 아직 없었다. 이에 저자들은 개에게서 얻은 벼룩을 대상으로 B. henselae DNA가 존재하는지 알아보기 위하여 본 연구를 시행하였다. 방 법 : 전체 22마리의 개에서 얻은 총 42마리의 벼룩을 대상으로 연구를 시행하였다. 각각의 벼룩에서 DNA를 추출한 다음 B. henselae의 pap31 부위 유전자를 이용한 시발체를 사용하여 seminested PCR을 시행하였다. 결 과 : B. henselae DNA는 전체 22마리 중 4마리의 개 (18.2%)에서 추출되었던 벼룩 42마리 중 7마리(16.7%)에서 확인되었다. PCR 결과를 확인하기 위하여 7개의 PCR 산물에 대한 염기서열 분석을 시행하였다. 염기서열 분석에 의하면 6개는 Huston-1 유전자형이었으며, 한 개는 Marseille 유전자형이었다.

다국어 초록 (Multilingual Abstract)

Purpose : The purpose of this study was to investigate the presence of Bartonella henselae DNA, which is known as an etiologic agent of lymphadenitis, in fleas from dogs.
Methods : The Bartonella henselae infection was investigated in 42 fleas from 22 dogs in Korea. By using seminested PCR targeting pap31 gene, B. henselae DNA was amplified from fleas.
Results : B. henselae DNA was detected in seven fleas (7 of 42 fleas, 16.7 percent) from four dogs (4 of 22 dogs, 18.2 percent). To confirm these findings, we performed sequencing and identified the seven PCR products. Sequence analysis revealed that six sequences belonged to Huston-1 genogroup and one sequence to Marseille genogroup.
Conclusion : These results may suggest that dogs could be an important source of B. henselae infection in children in Korea. This is the first report about the detection of B. henselae in fleas from dogs in Korea.

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