천연한방소재인 연교 추출물의 미백 효과에 관한 연구 = A Study on the Whitening Effect of the Oriental Medicinal Herb Forsythia suspensa Fruit as a Cosmetic Ingredient

다국어 초록 (Multilingual Abstract)

The aim of this study was to investigate the inhibitory effects of Forsythia suspensa fruit extracts (FSfE) (0.1~1.0%) and their active component on melanogenesis. FSfE dose-dependently inhibited melanin synthesis (up to 63.1±3.1% at the concentration of 1.0%) without cell cytotoxicity. We purifed one active compound from FSfE and identified its structure. It was identified as 4-[(3,4-Dimethoxyphenyl)methyl)]dihydro-3-[(4-hydroxy-3-methoxyphenyl)methyl]-2(3H)-furanone (arctigenin) by ¹H-NMR, ¹³C-NMR, and Mass analysis. Arctigenin also inhibited melanin synthesis in a dose dependent manner (up to 52.7±3.1% at the concentration of 3.0 μg/mL). In order to verify the whitening activity of the cream containing 3% FSfE, we performed the clinical test with twenty five female volunteers for 8 weeks. Bioengineering analyses and visual assessment by doctors at the initial time point and 8 weeks after applications showed that cream containing FSfE have significant whiteining effect (paired t-test). From the results, we conclude that the FSfE can be used as a useful whitening agent.

참고문헌 (Reference)

1 Maes, "Use of a chromameter in assessing the efficacy of anti-irritants and tanning accelerators" 1990

2 M. Seiberg, "The protease-activated receptor 2 regulates pigmentation via keratinocyte-melanocyte interaction" 254 : 25-, 2000

3 Kitagawa, "Studies on the chinese crude drug On isolation of phenylpropanoid glycosides from fruits of Forsythiae koreana and their antibacterial activity the Pharmaceutical Society of Japan" 1987

4 G. Prota, "Pigment cell research; what directions?" 10 : 5-, 1997

5 Lee, "Pharmacological studies on Forsythiae fructus" 1988

6 T. Horikoshi, "Involvement of nitric oxide in UVB-induced pigmentation in Guinea pig skin" 13 : 358-, 2000

7 T. Motogawa, "Inhibition of α-MSH induced melanogenesis by sophorae radix extracts" 9 : 38-, 2000

8 H. M. Aoki, "Identification of a new microphthalmia (Mi)-interacting protein, rKr2, involved in the regulation of melanogenesis" 1998

9 U. Yada, "Effects of endothelin on signal transduction and proliferation in human melanocytes" 266 (266): 18352-, 1997

10 R. Busca, "Cyclic AMP a key messenger in the regulation of skin pigmentation" 13 : 60-, 2000

11 K. Wakamatsu, "Characterisation of ACTH peptides in human skin and their activation of the melanocortin-1 receptor" 10 : 288-, 1997

12 J. H. Kim, "A caspase inducing inhibitor isolated from Forsythiae fructus" 34 (34): 114-, 2002