Identifying Markers Associated with Salinity Tolerance at Seedling Stage in Rice

다국어 초록 (Multilingual Abstract)

The F2 breeding population from a cross between IR73571-3B-11-3-K2 (Indica/japonica,
high tolerance) and Dongjinbyeo(Japonica, moderately susceptible) was screened for salinity tolerance at
seedling stage with electrical conductivity(EC) of 18 dSm-1. The frequency distribution of the salinity reaction
was continuous and skewed towards the susceptible parent Dongjinbyeo. Thirteen markers were saturated
with the target region of the chromosome 1. Two QTLs, qST-S1 and qST-S2, were detected found
to be related to salt tolerance at seedling stage on chromosome 1 with LOD 4.21 and 3.8 and explained
13% and 11.8% of phenotypic variations respectively. In allele effect of a2a2b1b2 and a1a2b2b2, the mean
of salt tolerance showed the difference between two QTLs. Thus, the linkage association on both the QTLs
might be too strong to get the recombination type, which could explain the independence of two QTLs.
The segregation distortion at 5% significant level was shown by eight markers of thirteen makers.
RM1287 located in segregation distortion part, whereas RM7075 didn't show segregation distortion.

참고문헌 (Reference)

1 Lee, K. S, "Variability and genetics of salt tolerance in Japonica rice(O. sativa L.)" University of Philippine 108-, 1995

2 Garcia, A, "The effects of selection for sodium transport and of selection for agronomic characteristics upon salt resistance in rice(Oryza sativa L.) Theor" 90 : 1106-1111, 1995

3 Gregorio G. B, "Tagging salinity tolerance genes in rice using amplified fragment length polymorphism(AFLP)" University of the Philippines Los Baños 118-, 1997

4 NICS(National Institute of Crop Science), "Stress tolerance breeding of rice in Korea" 111-, 2004

5 Gregorio, G. B, "Screening rice for salinity tolerance" (22) : 3-19, 1997

6 HARI(Honam Agriculture Research Institute), "Research for salinity tolerance in rice" 46-, 2004

7 Bonilla P. S, "RFLP and SSLP mapping of salinity tolerance genes in chromosome 1 of rice(Oryza sativa L.) using recombinantinbred lines" 85 (85): 64-74, 2002

8 Lang, N. T, "Quantitative trait loci for salt tolerance in rice via molecular markers" 8 : 37-48, 2000

9 Lin H. X, "QTLs for Na+ and K+ uptake of the shoots and roots controlling rice salt tolerance.Theor.Appl.Genet.108:253-260" 108 : 253-260, 2004

10 Basten, C., "QTL Cartographer. Department of Statistics" North Carolina State University 2001

11 Gregorio, G. B, "Progress in breeding for salinity tolerance and other abiotic associated stresses in rice. Field Crops Res"

12 McCouch, S. R, "Molecular mapping of rice chromosomes" 76 : 815-829, 1988

13 Islam,M.M, "Mappong salinity tolerance genes in rice(Oryza sativa L.) at reproductive stage" University of Philippine 150-, 2004

14 Tuan, V. D, "Mapping quantitative trait loci for salinity tolerance in rice" 8 : 27-35, 2000

15 Lin H. X, "Mapping of QTL for salt tolerance in rice(Oryza sativa L.) using molecular markers. Okinawa Subtropical Station" 4 : 240-265, 1995

16 Lander, E. S, "Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps" 21 : 185-199, 1989

17 Yoshida, S, "Laboratory manual for physiological studies of rice. International Rice Research Institute(IRRI), Los Banos, The Philippines"

18 Shahid Masood, M, "Identification and evaluation of salinity tolerance inf rice(O. sativa L.) using markers. Okinawa Subtropical Station" 6 : 121-135, 1997

19 Gregorio, G. B, "Genetics analysis of salinity tolerance in rice" 86 : 333-338, 1993

20 Niones, J. M, "Fine mapping of the salinity tolerance gene on chromosome 1 of rice(Oryza sativa L.) using near isogenic lines" University of the Philippines Los Baños 2004

21 Kurata, N, "A 300-kilobase interval genetic map of rice including 883 expressed sequences" 8 : 365-372, 1994